Zhang Sanyin, Yang Peng, Li Xixiong, Wang Xin, Song Jiage, Peng Wei, Wu Chunjie
Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China.
The Fifth People's Hospital of Chengdu, Chengdu, China.
Evid Based Complement Alternat Med. 2017;2017:1273561. doi: 10.1155/2017/1273561. Epub 2017 Dec 11.
To compare the effects of Semen Arecae (SA) and Charred Semen Arecae (CSA) on gastrointestinal motility, motilin, substance P (SP), and cholecystokinin (CCK) in chronically stressed rats.
Rats were randomly divided into control group and stress group. Rats in stress group were randomly exposed to a variety of unpredictable stimulations for 21 days. Then, the rats were treated orally with distilled water, SA, CSA, and mosapride for 7 days. Gastric residue rate and intestinal propulsion rate were evaluated. Serum levels of motilin and SP were measured by enzyme-linked immunosorbent assay (ELISA). CCK mRNA was quantified by using quantitative real-time PCR (qRT-PCR).
Both SA and CSA improved the intestinal propulsion and reduced the gastric residue in chronically stressed rats. Furthermore, the serum levels of motilin and SP were significantly higher and the CCK mRNA expressions in intestine and hypothalamus were downregulated in SA and CSA groups. Furthermore, it was found that CSA is more effective.
Both SA and CSA enhanced gastrointestinal motility and increased serum levels of motilin and SP in chronically stressed rats via downregulating CCK mRNA expressions in intestine and hypothalamus. Importantly, CSA possessed more effective promoting effects.
比较槟榔和炒槟榔对慢性应激大鼠胃肠动力、胃动素、P物质（SP）和胆囊收缩素（CCK）的影响。
将大鼠随机分为对照组和应激组。应激组大鼠随机接受各种不可预测的刺激21天。然后，大鼠分别灌胃蒸馏水、槟榔、炒槟榔和莫沙必利7天。评估胃残留率和肠推进率。采用酶联免疫吸附测定（ELISA）法检测胃动素和SP的血清水平。使用定量实时聚合酶链反应（qRT-PCR）对CCK mRNA进行定量。
槟榔和炒槟榔均能改善慢性应激大鼠的肠推进并减少胃残留。此外，槟榔和炒槟榔组大鼠血清胃动素和SP水平显著升高，小肠和下丘脑CCK mRNA表达下调。此外，发现炒槟榔效果更显著。
槟榔和炒槟榔均可通过下调小肠和下丘脑CCK mRNA表达增强慢性应激大鼠的胃肠动力并提高血清胃动素和SP水平。重要的是，炒槟榔具有更有效的促进作用。
