端粒酶RNA反义寡脱氧核苷酸对人结肠癌端粒酶活性及细胞凋亡的影响
Effect of antisense oligodeoxynucleotide of telomerase RNA on telomerase activity and cell apoptosis in human colon cancer.
作者信息
Jiang Ying-An, Luo He-Sheng, Fan Li-Fang, Jiang Chong-Qing, Chen Wei-Jin
机构信息
Department of Gastroenterology, Renming Hospital of Wuhan University, Hubei Province, China.
出版信息
World J Gastroenterol. 2004 Feb 1;10(3):443-5. doi: 10.3748/wjg.v10.i3.443.
AIM
To explore the effect of antisense oligodeoxynucleotide (As-ODN) of telomerase RNA on telomerase activity and cell apoptosis in human colon cancer.
METHODS
As-ODN was transfected into SW480 cells by liposomal transfection reagent. Telomerase activity of SW480 cells was examined by telomeric repeat amplification protocol (TRAP) and enzyme-linked immunosorbent assay (ELISA). Apoptosis was analyzed by morphology and flow cytometry.
RESULTS
The telomerase activity in SW480 cells transfected with 1.0 micromol/L of As-ODN for 2-5 days, was significantly decreased in a time-dependent manner, and the cells underwent apoptosis. The missense ODN (Ms-ODN) and the control group transfected with SW480 cells did not show these changes.
CONCLUSION
As-ODN can specifically inhibit the telomerase activity of SW480 cells and induce apoptosis.
目的
探讨端粒酶RNA反义寡脱氧核苷酸（As-ODN）对人结肠癌端粒酶活性及细胞凋亡的影响。
方法
采用脂质体转染试剂将As-ODN转染至SW480细胞。通过端粒重复序列扩增法（TRAP）和酶联免疫吸附测定（ELISA）检测SW480细胞的端粒酶活性。采用形态学和流式细胞术分析细胞凋亡情况。
结果
用1.0微摩尔/升As-ODN转染SW480细胞2至5天，端粒酶活性呈时间依赖性显著降低，细胞发生凋亡。错义寡脱氧核苷酸（Ms-ODN）组及转染SW480细胞的对照组未出现这些变化。
结论
As-ODN可特异性抑制SW480细胞的端粒酶活性并诱导细胞凋亡。
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