Erdemir Ali, Eldeniz Ayçe Unverdi, Belli Sema
Department of Endodontics, Selcuk University, Faculty of Dentistry, Konya, Turkey.
J Endod. 2004 Jan;30(1):54-6. doi: 10.1097/00004770-200401000-00012.
The purpose of this in vitro study was to evaluate the mineral contents of root-canal dentin before and after treatment with two commonly used gutta-percha solvents: chloroform and halothane. Twenty extracted human premolars, whose crowns and apical thirds had been removed, were used. Pulp tissues were removed and the teeth were randomly divided into two groups including 10 teeth each. Root canals were enlarged with Gates Glidden burs (#1, 2, and 3). Dentin chips were obtained and saved in plates to serve as a control. Root-canal-dentin walls were then treated with chloroform or halothane for 15 min. Dentin chips were again obtained using Gates Glidden burs (#4, 5, and 6). The levels of five elements, calcium, phosphorus, magnesium, potassium, and sulfur, in each specimen were analyzed using ICP-AES (inductively coupled plasma atomic emission spectrometry) technique. Changes in the levels of the chemical elements were recorded. Differences between the groups were statistically analyzed using Mann-Whitney U test. There was a significant decrease in Ca level and significant increase in Mg level after treatment with halothane (p < 0.05). There was a significant increase in Mg level after treatment with chloroform. The changes in other elements levels after treatment with gutta-percha solvents were minimal and statistically not significant (p > 0.05). As a result it was concluded that gutta-percha solvents have effect on mineral contents of root dentin.
氯仿和氟烷处理前后根管牙本质的矿物质含量。使用了20颗拔除的人类前磨牙，其冠部和根尖三分之一已被去除。去除牙髓组织，将牙齿随机分为两组，每组10颗。用盖茨·格利登钻针（#1、2和3）扩大根管。获取牙本质碎屑并保存在培养皿中作为对照。然后用氯仿或氟烷处理根管牙本质壁15分钟。再次用盖茨·格利登钻针（#4、5和6）获取牙本质碎屑。使用电感耦合等离子体原子发射光谱法（ICP-AES）技术分析每个样本中钙、磷、镁、钾和硫五种元素的含量。记录化学元素含量的变化。使用曼-惠特尼U检验对两组之间的差异进行统计学分析。用氟烷处理后，钙含量显著降低，镁含量显著升高（p<0.05）。用氯仿处理后，镁含量显著升高。用牙胶溶剂处理后其他元素含量的变化极小，且无统计学意义（p>0.05）。结果得出结论，牙胶溶剂对牙根牙本质的矿物质含量有影响。
