极低频电磁场对小鼠脑和肝细胞凋亡及细胞周期的影响

[Effects of extremely low frequency electromagnetic fields on apoptosis and cell cycle of mouse brain and liver cells].

作者信息

Liu Yun, Hong Rong, Yu Yun-mei, Weng En-qi

机构信息

Department of Environmental Science, East China Normal University, Shanghai 200062, China.

出版信息

Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2003 Oct;21(5):339-41.

PMID:14761394

Abstract

OBJECTIVE

To study the effects of extremely low frequency electromagnetic fields (ELF EMFs) on apoptosis and cell cycle of mouse brain and liver cells.

METHODS

Mice were exposed to 50 Hz, 0.2 mT or 6.0 mT electromagnetic fields for 2 weeks. TUNEL and flow cytometric methods were used to analyze apoptosis and cell cycle of brain and liver cells.

RESULTS

After exposure to 0.2 mT and 6.0 mT ELF EMFs for 2 weeks, apoptosis rates of brain cells [(5.60 +/- 1.47)% and (4.73 +/- 0.48)% respectively] were higher than that of control [(2.90 +/- 0.75)%], and apoptosis rates of liver cells [(4.19 +/- 2.08)% and (3.38 +/- 0.65)% respectively] were higher than that of control [(1.84 +/- 0.76)%]. G0/G1 cell percentage of brain cells [(80.21 +/- 1.68)% and (79.54 +/- 0.56)% respectively] were higher than that of control [(76.85 +/- 0.83)%], and those of liver cells [(79.42 +/- 1.80)% and (80.47 +/- 1.79)% respectively] were higher than that of control [(73.36 +/- 3.10)%]. The above differences were all statistically significant as P < 0.05. At the same time S and G2 + M cell percentage of brain and liver cells were significantly decreased.

CONCLUSION

Exposure to 50 Hz EMFs may alter cell cycle and induce apoptosis of mouse brain and liver cells.

摘要

目的

研究极低频电磁场（ELF EMFs）对小鼠脑和肝细胞凋亡及细胞周期的影响。

方法

将小鼠暴露于50Hz、0.2mT或6.0mT的电磁场中2周。采用TUNEL和流式细胞术方法分析脑和肝细胞的凋亡及细胞周期。

结果

暴露于0.2mT和6.0mT的ELF EMFs 2周后，脑细胞的凋亡率[分别为(5.60±1.47)%和(4.73±0.48)%]高于对照组[(2.90±0.75)%]，肝细胞的凋亡率[分别为(4.19±2.08)%和(3.38±0.65)%]高于对照组[(1.84±0.76)%]。脑细胞的G0/G1期细胞百分比[分别为(80.21±1.68)%和(79.54±0.56)%]高于对照组[(76.85±0.83)%]，肝细胞的G0/G1期细胞百分比[分别为(79.42±1.80)%和(80.47±1.79)%]高于对照组[(73.36±3.10)%]。上述差异均具有统计学意义（P<0.05）。同时，脑和肝细胞的S期及G2+M期细胞百分比显著降低。