极低频电磁场对小鼠雄性生殖的影响
[Effects of extremely low frequency electromagnetic fields on male reproduction in mice].
作者信息
Hong Rong, Liu Yun, Yu Yun-mei, Hu Ke, Weng En-qi
机构信息
Department of Environmental Science, East China Normal University, Shanghai 200062, China.
出版信息
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2003 Oct;21(5):342-5.
OBJECTIVE
To investigate the effects of extremely low frequency electromagnetic fields (ELF EMFs) on male reproduction in mice.
METHODS
94 adult male mice were exposed to 50 Hz sinusoidal electromagnetic fields of 0.2, 3.2 or 6.4 mT for 2 weeks or 4 weeks. Testicular histology and weight, sperm amount, sperm motility and morphology were measured. The percentages of different ploidy cells and cell phases, and DNA content of testis cells were estimated by flow cytometry. The micronucleus rate of bone-marrow cell was also observed.
RESULTS
The testicular weight of the mice exposed to 6.4 mT for 4 weeks [(76.06 +/- 32.25) mg] was significantly lower than that of the control [(111.44 +/- 19.99) mg, P < 0.05]; no significant histopathological changes were observed on the testis in EMFs exposed mice;the sperm amount was decreased after EMFs exposure for 4 weeks, and those of the mice exposed to 0.2 mT and 6.4 mT for 4 weeks [(4.87 +/- 0.94) x 10(6)/ml and (4.30 +/- 1.89) x 10(6)/ml respectively] were significantly lower than that of the control [(6.67 +/- 0.70) x 10(6)/ml, P < 0.05]; the rates of sperm motility also showed a decline. After 0.2, 3.2 or 6.4 mT EMFs exposure for 2 weeks, the deformity rates of sperm [(7.416 +/- 3.352)%, (6.862 +/- 2.947)% and (8.112 +/- 4.615)% respectively] were significantly higher than that of the control [(4.098 +/- 2.028)%, P < 0.01]. Similarly, those of the mice exposed for 4 weeks [(10.267 +/- 3.836)%, (11.027 +/- 7.059)%, (8.814 +/- 3.678)% respectively] were higher than that of the control [(3.714 +/- 1.830)%]. After 6.4 mT exposure for 2 weeks, the percentages of 1C testis cells [(69.56 +/- 4.07)%] was significantly lower than that of the control [(73.45 +/- 3.10)%, P < 0.05]. There were not any remarkable changes in those of 2C, 4C cells. DNA content in different ploidy cells of the mice exposed to 6.4 mT was decreased. Moreover, the cell percentage in S phase was increased significantly (P < 0.01).
CONCLUSION
ELF EMFs exposure may have some adverse effects on reproduction in mice.
目的
研究极低频电磁场（ELF EMFs）对小鼠雄性生殖功能的影响。
方法
将94只成年雄性小鼠暴露于0.2、3.2或6.4 mT的50 Hz正弦电磁场中2周或4周。测量睾丸组织学和重量、精子数量、精子活力和形态。通过流式细胞术估计不同倍体细胞和细胞周期的百分比以及睾丸细胞的DNA含量。同时观察骨髓细胞的微核率。
结果
暴露于6.4 mT 4周的小鼠睾丸重量[（76.06±32.25）mg]显著低于对照组[（111.44±19.99）mg，P<0.05]；暴露于电磁场的小鼠睾丸未观察到明显的组织病理学变化；暴露4周后精子数量减少，暴露于0.2 mT和6.4 mT 4周的小鼠精子数量[分别为（4.87±0.94）×10⁶/ml和（4.30±1.89）×10⁶/ml]显著低于对照组[（6.67±0.70）×10⁶/ml，P<0.05]；精子活力也呈下降趋势。暴露于0.2、3.2或6.4 mT电磁场2周后，精子畸形率[分别为（7.416±3.352）%、（6.862±2.947）%和（8.112±4.615）%]显著高于对照组[（4.098±2.028）%，P<0.01]。同样，暴露4周的小鼠[分别为（10.267±3.836）%、（11.027±7.059）%、（8.814±3.678）%]高于对照组[（3.714±1.830）%]。暴露于6.4 mT 2周后，1C睾丸细胞百分比[（69.56±4.07）%]显著低于对照组[（73.45±3.10）%，P<0.05]。2C、4C细胞百分比无明显变化。暴露于6.4 mT的小鼠不同倍体细胞的DNA含量降低。此外，S期细胞百分比显著增加（P<0.01）。
结论
暴露于极低频电磁场可能对小鼠生殖功能产生一些不良影响。
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