Hong Rong, Zhang Yan, Liu Yun, Weng En-qi
Department of Environment Science, East China Normal University, Shanghai 200062, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2005 Dec;23(6):414-7.
To study the effects of 50 Hz electromagnetic fields (EMFs) on DNA of testicular cells and sperm chromatin structure in mice.
Mice were exposed to 50 Hz, 0.2 mT or 6.4 mT electromagnetic fields for 4 weeks. DNA strand breakage in testicular cells was detected by single-cell gel electrophoresis assay. Sperm chromatin structure was analyzed by sperm chromatin structure assay with flow cytometry.
After 50 Hz, 0.2 mT or 6.4 mT EMFs exposure, the percentage of cells with DNA migration in total testicular cells increased from the control level of 25.64% to 37.83% and 39.38% respectively. The relative length of comet tail and the percentage of DNA in comet tail respectively increased from the control levels of 13.06% +/- 12.38% and 1.52% +/- 3.25% to 17.86% +/- 14.60% and 2.32% +/- 4.26% after 0.2 mT exposure and to 17.88% +/- 13.71% and 2.35% +/- 3.87% after 6.4 mT exposure (P < 0.05). Exposure to EMFs had not induced significant changes in S.D.alphaT and XalphaT, but COMPalphaT (cells outside the main population of alpha t), the percentage of sperms with abnormal chromatin structure, increased in the two exposed groups.
50 Hz EMFs may have the potential to induce DNA strand breakage in testicular cells and sperm chromatin condensation in mice.
研究50赫兹电磁场（EMF）对小鼠睾丸细胞DNA及精子染色质结构的影响。
将小鼠暴露于50赫兹、0.2毫特斯拉或6.4毫特斯拉的电磁场中4周。采用单细胞凝胶电泳法检测睾丸细胞中的DNA链断裂情况。运用流式细胞术通过精子染色质结构分析法分析精子染色质结构。
在暴露于50赫兹、0.2毫特斯拉或6.4毫特斯拉的电磁场后，睾丸细胞中出现DNA迁移的细胞百分比分别从对照组的25.64%增至37.83%和39.38%。在0.2毫特斯拉暴露后，彗星尾相对长度及彗星尾中DNA百分比分别从对照组的13.06%±12.38%和1.52%±3.25%增至17.86%±14.60%和2.32%±4.26%，在6.4毫特斯拉暴露后分别增至17.88%±13.71%和2.35%±3.87%（P<0.05）。暴露于电磁场未引起S.D.αT和XαT的显著变化，但在两个暴露组中，COMPαT（αt主要群体之外的细胞），即染色质结构异常的精子百分比增加。
50赫兹电磁场可能有诱导小鼠睾丸细胞DNA链断裂及精子染色质凝聚的潜在作用。
