Campa Cristiana, Oust Astrid, Skjåk-Braek Gudmund, Paulsen Berit Smestad, Paoletti Sergio, Christensen Bjørn E, Ballance Simon
Department of Biochemistry, Biophysics and Macromolecular Chemistry University of Trieste, 1-34127 Trieste, Italy.
J Chromatogr A. 2004 Feb 13;1026(1-2):271-81. doi: 10.1016/j.chroma.2003.11.045.
The average degree of polymerisation (DP) and distribution of oligosaccharides in partially acid hydrolysed mannuronans were quantitatively evaluated by 1H NMR, electrospray ionisation mass spectrometry (ESI-MS), micellar electrokinetic capillary chromatography with UV detection (MEKC-UV), and high-pressure anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD). Our investigation shows that 1H NMR, MEKC-UV and, in particular, HPAEC-PAD can be used as quantitative tools to aid the investigation of polysaccharide structure, function and synthesis. For the latter two techniques, especially, this represents a significant new development as it enables calculation of the quantity of individual oligomers of nominal DP by direct analysis of a defined oligomer mixture. Appropriate statistical averages of number and weight distributions were also calculated and found to fit very well to predicted Kuhn distributions that assume random depolymerisation.
通过1H核磁共振、电喷雾电离质谱（ESI-MS）、带紫外检测的胶束电动毛细管色谱法（MEKC-UV）以及带脉冲安培检测的高压阴离子交换色谱法（HPAEC-PAD），对部分酸水解甘露聚糖中低聚糖的平均聚合度（DP）和分布进行了定量评估。我们的研究表明，1H核磁共振、MEKC-UV，尤其是HPAEC-PAD可作为定量工具，辅助多糖结构、功能和合成的研究。特别是对于后两种技术而言，这是一项重大的新进展，因为它能够通过直接分析特定的低聚物混合物来计算标称DP的单个低聚物的数量。还计算了数量和重量分布的适当统计平均值，发现其与假设随机解聚的预测库恩分布非常吻合。
