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Simplified and rapid method for extraction of ergosterol from natural samples and detection with quantitative and semi-quantitative methods using thin-layer chromatography.

作者信息

Larsen Thomas, Axelsen Jørgen, Weber Ravn Helle

机构信息

Department of Terrestrial Ecology, National Environmental Research Institute, Vejlsøvej 25, DK-8600 Silkeborg, Denmark.

出版信息

J Chromatogr A. 2004 Feb 13;1026(1-2):301-4. doi: 10.1016/j.chroma.2003.10.128.

DOI:10.1016/j.chroma.2003.10.128
PMID:14763758
Abstract

A new and simplified method for extraction of ergosterol (ergosta-5,7,22-trien-3beta-ol) from fungi in soil and litter was developed using pre-soaking extraction and paraffin oil for recovery. Recoveries of ergosterol were in the range of 94-100% depending on the solvent to oil ratio. Extraction efficiencies equal to heat-assisted extraction treatments were obtained with pre-soaking extraction. Ergosterol was detected with thin-layer chromatography (TLC) using fluorodensitometry with a quantification limit of 8 ng. Using visual evaluation of images of TLC plates photographed in UV-light the quantification limit was 16 ng.

摘要

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