Applicability of an immunoblot technique combined with a semiautomated electrophoresis system for detection of staphylococcal enterotoxins in food extracts.
作者信息
Orden J A, Goyache J, Hernández J, Doménech A, Suárez G, Gómez-Lucía E
机构信息
Departamento de Patologia Animal I, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
出版信息
Appl Environ Microbiol. 1992 Dec;58(12):4083-5. doi: 10.1128/aem.58.12.4083-4085.1992.
Abstract
We studied the usefulness of an immunoblot technique for the detection of staphylococcal enterotoxins (SEs) in strains and food extracts. Food samples (milk, yogurt, hot dog sausage, cheese, and mayonnaise) were artificially contaminated with SEA through SEE. Protein A did not interfere with the results; it appeared on electrophoresis gels as bands with molecular weights higher than those of the SEs. Other food proteins were not revealed by the technique. The immunoblot technique proved to be fast, specific, and sensitive for the detection of SEs in foods.
摘要
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