Garaulet Marta, Viguerie Nathalie, Porubsky Stefan, Klimcakova Eva, Clement Karine, Langin Dominique, Stich Vladimir
Franco-Czech Laboratory for Clinical Research on Obesity, French Institute of Health and Medical Research, Institut National de la Santé et d la Recherche Médicale U586 and 3rd Faculty of Medicine, Charles University, Prague, Czech Republic.
J Clin Endocrinol Metab. 2004 Feb;89(2):756-60. doi: 10.1210/jc.2003-031495.
Adiponectin, a newly discovered adipose-tissue-specific protein, is thought to be involved in the regulation of insulin action. The aim of the present study was to determine whether adiponectin contributes to the improvement in insulin sensitivity during very-low-calorie diet (VLCD). Biopsies of sc abdominal adipose tissue and blood sampling for analysis of plasma adiponectin and related hormones and metabolites were performed before and at the end of a 4-wk VLCD in 33 nonmorbidly obese women (body mass index, 34.4 +/- 4.1 kg/m(2)). VLCD produced a decrease in weight (7.1 +/- 0.4 kg) and in insulin and leptin levels and led to an improvement in insulin sensitivity. Adiponectin gene expression and plasma levels were not modified during calorie restriction. Before VLCD, we found negative correlations between plasma adiponectin and variables related to the metabolic syndrome. Adiponectin mRNA levels showed a negative correlation with lipoprotein a plasma values. The correlations observed before VLCD were not found after VLCD. The data suggest that adiponectin is related to the protection against the metabolic syndrome but is not involved in the regulation of VLCD-induced improvement of insulin sensitivity.
脂联素是一种新发现的脂肪组织特异性蛋白，被认为参与胰岛素作用的调节。本研究的目的是确定脂联素是否有助于极低热量饮食（VLCD）期间胰岛素敏感性的改善。对33名非病态肥胖女性（体重指数，34.4±4.1kg/m²）在4周VLCD开始前和结束时进行腹部皮下脂肪组织活检及血样采集，以分析血浆脂联素及相关激素和代谢产物。VLCD导致体重减轻（7.1±0.4kg）、胰岛素和瘦素水平降低，并使胰岛素敏感性得到改善。在热量限制期间，脂联素基因表达和血浆水平未发生改变。在VLCD前，我们发现血浆脂联素与代谢综合征相关变量之间存在负相关。脂联素mRNA水平与血浆脂蛋白a值呈负相关。VLCD后未发现VLCD前观察到的相关性。数据表明，脂联素与预防代谢综合征有关，但不参与调节VLCD诱导的胰岛素敏感性改善。
