Kim M J, Maachi M, Debard C, Loizon E, Clément K, Bruckert E, Hainque B, Capeau J, Vidal H, Bastard J P
INSERM U-680, Faculté de Médecine, Université Pierre et Marie Curie, 75012 Paris, France.
Eur J Endocrinol. 2006 Jul;155(1):161-5. doi: 10.1530/eje.1.02194.
To investigate the mRNA expression of adiponectin, AdipoR1 and AdipoR2, the two recently cloned adiponectin receptors and peroxisome proliferator activated receptor (PPAR)gamma2 in adipose tissue of obese individuals before and during a very low calorie diet (VLCD) inducing weight loss.
Twenty-three non-diabetic obese subjects with normal (NGT, n = 11) or impaired glucose tolerance (IGT, n = 12) (age, 47 +/- 3 years; body mass index, 39.3 +/- 1.3 kg/m2) were studied before and after a 3-week 3.9 MJ diet daily without exercise. mRNA levels of nine IGT and six NGT subjects were measured by real-time PCR in s.c. abdominal adipose tissue.
Metabolic parameters and insulin sensitivity were improved by VLCD in the IGT group, but minimally affected in the NGT group. VLCD increased expression of AdipoR1 in the IGT (P = 0.02), but not in the NGT group. Adiponectin, AdipoR2 and PPARgamma2 mRNA levels did not change during VLCD in any group. In the IGT, but not in the NGT group, AdipoR1 and AdipoR2 expressions were positively related to that of PPARgamma2 and, after VLCD, AdipoR1 and AdipoR2 expressions were positively related to each other and to that of adiponectin.
In the NGT group, the 3-week VLCD inducing weight loss did not modify metabolic parameters, insulin sensitivity and the expression of the adiponectin system in adipose tissue. By contrast, in the IGT group, AdipoR1 expression increased and we found a coordinate regulation of the expression of adiponectin and its receptors. These modifications could participate, through adiponectin action on adipocytes, to the improved metabolic parameters observed in IGT subjects.
研究脂联素、最近克隆的两种脂联素受体AdipoR1和AdipoR2以及过氧化物酶体增殖物激活受体（PPAR）γ2在肥胖个体极低热量饮食（VLCD）诱导体重减轻之前和期间的脂肪组织中的mRNA表达。
对23名非糖尿病肥胖受试者进行研究，其中11名糖耐量正常（NGT），12名糖耐量受损（IGT）（年龄47±3岁；体重指数39.3±1.3kg/m²）。在每日3.9MJ饮食且不运动的3周前后对他们进行研究。通过实时PCR测量9名IGT和6名NGT受试者腹部皮下脂肪组织中的mRNA水平。
VLCD使IGT组的代谢参数和胰岛素敏感性得到改善，但对NGT组影响极小。VLCD使IGT组中AdipoR1的表达增加（P = 0.02），但NGT组中未增加。任何组在VLCD期间脂联素、AdipoR2和PPARγ2的mRNA水平均未改变。在IGT组而非NGT组中，AdipoR1和AdipoR2的表达与PPARγ2的表达呈正相关，且在VLCD后，AdipoR1和AdipoR2的表达彼此呈正相关并与脂联素的表达呈正相关。
在NGT组中，为期3周的VLCD诱导体重减轻并未改变代谢参数、胰岛素敏感性以及脂肪组织中脂联素系统的表达。相比之下，在IGT组中，AdipoR1表达增加，并且我们发现脂联素及其受体的表达存在协同调节。这些改变可能通过脂联素对脂肪细胞的作用，参与了IGT受试者中观察到的代谢参数改善。
