Caroci A S, Calvo E, Ribolla P E, De Bianchi A G, Marinotti O
Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP, CEP 05,508-900, Brazil.
J Med Entomol. 2003 Nov;40(6):991-5. doi: 10.1603/0022-2585-40.6.991.
Trypsin activity increases in the midgut of Anopheles aquasalis, Anopheles albitarsis, and Anopheles darlingi after a bloodmeal. The activity returns to basal levels at the time the blood is completely digested. Affinity chromatography, reversed-phase high performance liquid chromatography (HPLC), and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) were used to sequentially purify the mosquito trypsins found in the midguts at 24 h after feeding. Aminoterminal sequencing of the purified trypsins showed the occurrence of two distinct trypsins in the midgut of each of the mosquitoes studied. The sequences obtained are similar to those of the trypsins of other hematophagous insects.
饱血后，嗜人按蚊、白跗按蚊和达林按蚊中肠的胰蛋白酶活性增加。当血液完全消化时，该活性恢复到基础水平。采用亲和色谱、反相高效液相色谱（HPLC）和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）对进食后24小时中肠中发现的蚊子胰蛋白酶进行了顺序纯化。纯化胰蛋白酶的氨基末端测序表明，在所研究的每只蚊子的中肠中都存在两种不同的胰蛋白酶。获得的序列与其他吸血昆虫的胰蛋白酶序列相似。
