Dias-Lopes Geovane, Borges-Veloso Andre, Saboia-Vahia Leonardo, Padrón Gabriel, de Faria Castro Cássia Luana, Guimarães Ana Carolina Ramos, Britto Constança, Cuervo Patricia, De Jesus Jose Batista
Laboratório de Biologia Molecular e Doenças Endêmicas, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brazil.
Laboratório de Pesquisa em Leishmaniose, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brazil.
Parasitol Res. 2016 May;115(5):1977-89. doi: 10.1007/s00436-016-4940-6. Epub 2016 Feb 6.
Anopheles (Nyssorhynchus) aquasalis is a malaria vector mainly distributed along the coastal regions of South and Central America. In the absence of an effective vaccine against malaria, strategies for controlling the vector are the main tool for interrupting parasite transmission. Mechanisms of oogenesis and embryogenesis in anautogenous mosquitoes are mainly modulated by blood feeding. However, the expression, at the protein level, of genes involved in such mechanisms in sugar-fed females is unknown. In this work, total protein extracts of the reproductive tract of female An. aquasalis that were fed sugar were analyzed using liquid chromatography followed by mass spectrometry for protein identification and bioinformatic tools for data mining. We identified 922 proteins expressed in the organ, and using several databases, we attributed biological meaning for several of them. Remarkably, nine proteins involved in oogenesis were identified in females fed sugar. Putative vitellogenins, vitellogenin receptor, lipid storage droplet, transferrin, ferritin, and apolipoprotein, identified here, are proteins involved in egg development. Proteins involved in embryonic development, such as paxillin, exuperantia, several growth factors, and dorsal switch protein, were identified. Interestingly, in this study, we identified 15 peptidases of various classes such as aminopeptidases, carboxypeptidases, serine protease, cathepsin, and metalloprotease that could potentially interact with male seminal components. Here, we demonstrated that the reproductive tract of female An. aquasalis fed on sugar expresses proteins involved in oogenesis and embryonic development. These findings reveal unknown aspects of the physiology of this organ under the given nutritional conditions.
黄热按蚊是一种主要分布在南美洲和中美洲沿海地区的疟疾传播媒介。在缺乏有效的疟疾疫苗的情况下，控制传播媒介的策略是阻断寄生虫传播的主要手段。非自育性蚊子的卵子发生和胚胎发生机制主要受吸血调节。然而，在以糖为食的雌蚊中，参与这些机制的基因在蛋白质水平上的表达尚不清楚。在这项研究中，我们对以糖为食的雌性黄热按蚊生殖道的总蛋白提取物进行了液相色谱分析，随后通过质谱进行蛋白质鉴定，并使用生物信息学工具进行数据挖掘。我们鉴定出该器官中表达的922种蛋白质，并利用多个数据库为其中几种蛋白质赋予了生物学意义。值得注意的是，在以糖为食的雌蚊中鉴定出了9种参与卵子发生的蛋白质。这里鉴定出的假定卵黄原蛋白、卵黄原蛋白受体、脂质储存小滴、转铁蛋白、铁蛋白和载脂蛋白是参与卵子发育的蛋白质。还鉴定出了参与胚胎发育的蛋白质，如桩蛋白、丰盛蛋白、几种生长因子和背侧开关蛋白。有趣的是，在本研究中，我们鉴定出了15种不同类型的肽酶，如氨肽酶、羧肽酶、丝氨酸蛋白酶、组织蛋白酶和金属蛋白酶，它们可能与雄性精液成分相互作用。在这里，我们证明了以糖为食的雌性黄热按蚊生殖道表达参与卵子发生和胚胎发育的蛋白质。这些发现揭示了在给定营养条件下该器官生理学中未知的方面。
