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秸秆组分和储存方法对酶解过程中葡萄糖生成的影响。

Effect of stover fraction and storage method on glucose production during enzymatic hydrolysis.

作者信息

Montross M D, Crofcheck C L

机构信息

Department of Biosystems and Agricultural Engineering, University of Kentucky, 128 CE Barnhart Building, Lexington, KY 40546, USA.

出版信息

Bioresour Technol. 2004 May;92(3):269-74. doi: 10.1016/j.biortech.2003.09.007.

DOI:10.1016/j.biortech.2003.09.007
PMID:14766160
Abstract

One avenue for overcoming the economic challenges associated with the production of ethanol from renewable resources is to reduce the cost of the biomass feedstock. The balance between storage costs and benefits depend on the storage method and composition changes of individual stover fractions. Corn stover from bales stored inside and outside of a barn was separated into an interior and exterior layer after approximately 10 months of storage. The cobs, stalks, and leaves and husks were separated, dried, and ground through a 2 mm screen. Stover, sodium acetate (buffer), cellulase, and deionized water were added to 125 ml flasks. The mixture was held at 50 degrees C in an incubator and samples taken for glucose determination. The average glucose concentration after 60 h of hydrolysis from cobs, leaves and husks, and stalks was 10.5, 9.6, and 3.1 g/l, respectively. Cobs, leaves, and husks produced over 300% more glucose than stalks. Storage outside of the barn decreased the glucose production from individual stover components between 4% and 8%. The effect of stover fraction type on glucose production was significant, while the storage treatment effect was not significant. Fractionation of corn stover may be a method to increase the value of corn stover as a feedstock for glucose production.

摘要

克服与利用可再生资源生产乙醇相关经济挑战的一条途径是降低生物质原料的成本。储存成本与效益之间的平衡取决于储存方法以及各个秸秆组分的成分变化。在谷仓内外储存的成捆玉米秸秆在储存约10个月后被分成内层和外层。将玉米芯、秸秆、叶子和外皮分离、干燥并通过2毫米筛网研磨。将秸秆、醋酸钠(缓冲液)、纤维素酶和去离子水加入到125毫升烧瓶中。混合物在培养箱中保持在50摄氏度,并取样测定葡萄糖含量。玉米芯、叶子和外皮以及秸秆在水解60小时后的平均葡萄糖浓度分别为10.5克/升、9.6克/升和3.1克/升。玉米芯、叶子和外皮产生的葡萄糖比秸秆多300%以上。在谷仓外储存使各个秸秆组分的葡萄糖产量降低了4%至8%。秸秆组分类型对葡萄糖产量的影响显著,而储存处理的影响不显著。玉米秸秆的分级分离可能是一种提高玉米秸秆作为葡萄糖生产原料价值的方法。

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