Li Shuanfang, Miner Kelly, Fannin Rick, Carl Barrett J, Davis Barbara J
Laboratory of Women's Health, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA.
Gynecol Oncol. 2004 Feb;92(2):622-7. doi: 10.1016/j.ygyno.2003.10.053.
Cyclooxygenase-1 and 2 (COX-1 and COX-2) play important roles in normal physiology and are often dysregulated in neoplastic tissues. The present study determines whether COX-1 and COX-2 are expressed in ovarian cancers and whether the pattern of expression of these enzymes reveals clues to their roles in this cancer.
The expression of COX-1 and COX-2 proteins in 9 normal human ovaries, in 137 cases of ovarian cancers of epithelial origin (83 primary and 54 metastatic), and in 7 ovarian cancer cell lines was examined by immunohistochemistry and western analysis.
COX-1 protein was present in 95/137 (69.3%) of the total cancers studied, with 55/83 (66.3%) of the primary cancers and 40/54 (74.1%) of the metastatic cancers positive for protein. COX-2 was present in 97/137 (70.8%) of all cancers studied, with 53/83 (63.9%) of the primary cancers and 44/54 (81.5%) of the metastatic cancers positive for protein. Notably, the quickscores for COX-2-positive staining were significantly higher in metastatic cancers. Moreover, COX-2 immunostaining was frequently found at the advancing margin of tumor invasion or in new metastatic loci. COX-1 protein expression was observed in the ovarian surface epithelial cells, especially that of the inclusion cysts. COX-1 was also detected by western blot in seven of nine ovarian cancer cell lines. However, no COX-2 was detected in either normal epithelium or cancer cell lines.
COX-1 and COX-2 were expressed in every type of ovarian epithelial cancer, suggesting that each may contribute to the cancer development or progression.
环氧化酶-1和2（COX-1和COX-2）在正常生理过程中发挥重要作用，且在肿瘤组织中常出现失调。本研究旨在确定COX-1和COX-2在卵巢癌中是否表达，以及这些酶的表达模式是否能揭示它们在这种癌症中的作用线索。
采用免疫组织化学和蛋白质印迹分析，检测9例正常人类卵巢、137例上皮性卵巢癌（83例原发性和54例转移性）以及7种卵巢癌细胞系中COX-1和COX-2蛋白的表达情况。
在所研究的全部癌症中，95/137（69.3%）表达COX-1蛋白，其中原发性癌症55/83（66.3%）、转移性癌症40/54（74.1%）呈蛋白阳性。在所有研究的癌症中，97/137（70.8%）表达COX-2蛋白，其中原发性癌症53/83（63.9%）、转移性癌症44/54（81.5%）呈蛋白阳性。值得注意的是，转移性癌症中COX-2阳性染色的快速评分显著更高。此外，COX-2免疫染色常见于肿瘤侵袭的前沿或新的转移部位。在卵巢表面上皮细胞，尤其是包涵囊肿的上皮细胞中观察到COX-1蛋白表达。蛋白质印迹法在9种卵巢癌细胞系中的7种中也检测到了COX-1。然而，在正常上皮或癌细胞系中均未检测到COX-2。
COX-1和COX-2在每种类型的卵巢上皮癌中均有表达，提示它们可能各自对癌症的发生或进展有促进作用。
