Schneller M, Geiger R E
Abteilung für Klinische Chemie, Universität München.
Biol Chem Hoppe Seyler. 1992 Nov;373(11):1095-104. doi: 10.1515/bchm3.1992.373.2.1095.
A highly effective method for the synthesis of neoglycoconjugates has been developed. The technique is based on two steps. First, a sulfhydryl group is introduced into the carbohydrate by reductive amination with cysteamine, and secondly the sulfhydryl group carrying carbohydrate is coupled to a protein or carrier via a disulfide bridge. The method was used to couple sialic acid-containing carbohydrates such as N-acetylneuraminyllactose and the carbohydrate moieties of gangliosides (GM1, GM3 and GD1b) to bovine serum albumin. Biological functionality of the generated GM1- and GD1b-neoglycoprotein was demonstrated by a choleratoxin-binding assay and an immunoassay, respectively. The applicability of neoglycoconjugates for the quantification of gangliosides in solution by a competitive assay is shown.
已开发出一种合成新糖缀合物的高效方法。该技术基于两个步骤。首先，通过与半胱胺的还原胺化反应将巯基引入碳水化合物中，其次，携带碳水化合物的巯基通过二硫键与蛋白质或载体偶联。该方法用于将含唾液酸的碳水化合物，如N-乙酰神经氨酸乳糖和神经节苷脂（GM1、GM3和GD1b）的碳水化合物部分与牛血清白蛋白偶联。分别通过霍乱毒素结合试验和免疫试验证明了所生成的GM1-和GD1b-新糖蛋白的生物学功能。展示了新糖缀合物通过竞争试验定量溶液中神经节苷脂的适用性。
