通过暴露于极端酸性条件改善对肠出血性大肠杆菌的实验室富集。
Improved laboratory enrichment for enterohemorrhagic Escherichia coli by exposure to extremely acidic conditions.
作者信息
Grant Michael A
机构信息
United States Food and Drug Administration, Bothell, Washington 98021, USA.
出版信息
Appl Environ Microbiol. 2004 Feb;70(2):1226-30. doi: 10.1128/AEM.70.2.1226-1230.2004.
Abstract
Analysis of food samples for E. coli O157:H7 using the standard U.S. Food and Drug Administration procedure is frequently complicated by overgrowth of nontarget microorganisms. A new procedure was developed for enrichment of enterohemorrhagic E. coli (EHEC) which utilizes exposure to pH 2.00 for 2 h. This procedure yielded larger populations of EHEC than the standard method by factors ranging from 2.7 to 7.7 and, when age-stressed cultures were used, by factors ranging from 2.7 to 11.5. Cultures of competing enterics were more effectively inhibited by the new enrichment protocol as well.
摘要
采用美国食品药品监督管理局的标准程序对食品样本进行大肠杆菌O157:H7分析时，非目标微生物的过度生长常常使分析过程变得复杂。开发了一种新的程序用于富集肠出血性大肠杆菌（EHEC），该程序利用将样本暴露于pH 2.00的环境中2小时。与标准方法相比，此程序培养出的EHEC数量更多，倍数在2.7至7.7之间；若使用老化应激培养物，则倍数在2.7至11.5之间。新的富集方案对竞争性肠道菌培养物的抑制效果也更佳。
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