城市原生污水和处理后污水中肠出血性大肠杆菌的流行情况。
Prevalence of enterohemorrhagic Escherichia coli in raw and treated municipal sewage.
作者信息
Grant S B, Pendroy C P, Mayer C L, Bellin J K, Palmer C J
机构信息
Department of Civil and Environmental Engineering, University of California, Irvine 92717, USA.
出版信息
Appl Environ Microbiol. 1996 Sep;62(9):3466-9. doi: 10.1128/aem.62.9.3466-3469.1996.
Abstract
Municipal sewage was screened for DNA encoding Shiga-like Toxin (SLT) II, a key protein involved in the virulence of enterohemorrhagic Escherichia coli. PCR analysis of sewage concentrates showed that DNA encoding SLT II was present in a single sample of untreated sewage and absent in all other samples tested (n = 6). Thermotolerant E. coli cultured from the sewage (n = 1,520) also tested negative for SLT II by colony hybridization.
摘要
对城市污水进行了检测，以寻找编码志贺样毒素（SLT）II的DNA，SLT II是肠出血性大肠杆菌致病性的关键蛋白。对污水浓缩物进行的PCR分析表明，编码SLT II的DNA存在于一份未经处理的污水样本中，而在所有其他测试样本（n = 6）中均未检测到。从污水中培养出的耐热大肠杆菌（n = 1520）通过菌落杂交检测SLT II也呈阴性。
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