通过对革兰氏染色材料进行直接基因分型分析，在一名重症人类免疫缺陷病毒感染患者中鉴定出百日咳博德特氏菌，并利用独特的recA基因限制性酶切位点与霍氏博德特氏菌进行区分。
Identification of Bordetella pertussis in a critically ill human immunodeficiency virus-infected patient by direct genotypical analysis of Gram-stained material and discrimination from B. holmesii by using a unique recA gene restriction enzyme site.
作者信息
Vielemeyer Ole, Crouch Jill Y, Edberg Stephen C, Howe John G
机构信息
Department of Laboratory Medicine, Yale University School of Medicine, New Haven, Connecticut 06520-8035, USA.
出版信息
J Clin Microbiol. 2004 Feb;42(2):847-9. doi: 10.1128/JCM.42.2.847-849.2004.
Abstract
Bordetella pertussis was diagnosed in a human immunodeficiency virus-infected patient by a newly developed method in which bacterial DNA is amplified directly from sputum Gram-stained slides. The validation of the method is described along with an additional new PCR-based assay that can distinguish between B. pertussis and Bordetella holmesii.
摘要
通过一种新开发的方法，即直接从痰革兰氏染色玻片上扩增细菌DNA，在一名感染人类免疫缺陷病毒的患者中诊断出百日咳博德特氏菌。文中描述了该方法的验证过程以及另一种基于聚合酶链反应的新检测方法，该方法可以区分百日咳博德特氏菌和霍氏博德特氏菌。
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Identification of Bordetella pertussis in a critically ill human immunodeficiency virus-infected patient by direct genotypical analysis of Gram-stained material and discrimination from B. holmesii by using a unique recA gene restriction enzyme site.通过对革兰氏染色材料进行直接基因分型分析，在一名重症人类免疫缺陷病毒感染患者中鉴定出百日咳博德特氏菌，并利用独特的recA基因限制性酶切位点与霍氏博德特氏菌进行区分。
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