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Identification of Bordetella pertussis in a critically ill human immunodeficiency virus-infected patient by direct genotypical analysis of Gram-stained material and discrimination from B. holmesii by using a unique recA gene restriction enzyme site.通过对革兰氏染色材料进行直接基因分型分析，在一名重症人类免疫缺陷病毒感染患者中鉴定出百日咳博德特氏菌，并利用独特的recA基因限制性酶切位点与霍氏博德特氏菌进行区分。
J Clin Microbiol. 2004 Feb;42(2):847-9. doi: 10.1128/JCM.42.2.847-849.2004.
2
Bordetella holmesii DNA is not detected in nasopharyngeal swabs from Finnish and Dutch patients with suspected pertussis.在芬兰和荷兰疑似百日咳患者的鼻咽拭子中未检测到霍氏博德特氏菌DNA。
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3
Epidemiology of pertussis in Casablanca (Morocco): contribution of conventional and molecular diagnosis tools.卡萨布兰卡（摩洛哥）百日咳的流行病学：传统诊断工具与分子诊断工具的贡献
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Bordetella holmesii: Causative agent of pertussis.支气管败血波氏杆菌：百日咳的病原体。
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Diagnostic evaluation of polymerase chain reaction discriminative for Bordetella pertussis, B. parapertussis, and B. bronchiseptica.用于鉴别百日咳博德特氏菌、副百日咳博德特氏菌和支气管败血博德特氏菌的聚合酶链反应诊断评估
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Prevalence of Bordetella pertussis and Bordetella parapertussis infections in Tunisian hospitalized infants: results of a 4-year prospective study.在突尼斯住院婴儿中，百日咳博德特氏菌和副百日咳博德特氏菌感染的流行情况：一项为期 4 年的前瞻性研究结果。
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Improving specificity of Bordetella pertussis detection using a four target real-time PCR.使用四靶点实时荧光定量PCR提高百日咳博德特氏菌检测的特异性
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Recovery of Bordetella holmesii from patients with pertussis-like symptoms: use of pulsed-field gel electrophoresis to characterize circulating strains.从有百日咳样症状的患者中分离出霍氏博德特菌：使用脉冲场凝胶电泳对流行菌株进行特征分析。
J Clin Microbiol. 2000 Jun;38(6):2330-3. doi: 10.1128/JCM.38.6.2330-2333.2000.

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A real-time PCR assay with improved specificity for detection and discrimination of all clinically relevant Bordetella species by the presence and distribution of three Insertion Sequence elements.一种实时聚合酶链反应检测方法，通过三种插入序列元件的存在和分布，提高了对所有临床相关博德特氏菌属物种的检测和鉴别特异性。
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Novel duplex real-time PCR assay detects Bordetella holmesii in specimens from patients with Pertussis-like symptoms in Ontario, Canada.新型双重实时 PCR 检测法可检测加拿大安大略省类百日咳症状患者标本中的博德特氏霍氏菌。
J Clin Microbiol. 2010 Apr;48(4):1435-7. doi: 10.1128/JCM.02417-09. Epub 2010 Feb 24.
7
Multitarget PCR for diagnosis of pertussis and its clinical implications.用于百日咳诊断的多重PCR及其临床意义。
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Molecular pathogenesis, epidemiology, and clinical manifestations of respiratory infections due to Bordetella pertussis and other Bordetella subspecies.百日咳博德特氏菌及其他博德特氏菌亚种引起的呼吸道感染的分子发病机制、流行病学及临床表现
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本文引用的文献

1
PCR-based detection and identification of Burkholderia cepacia complex pathogens in sputum from cystic fibrosis patients.基于聚合酶链反应（PCR）检测和鉴定囊性纤维化患者痰液中的洋葱伯克霍尔德菌复合群病原体。
J Clin Microbiol. 2001 Dec;39(12):4247-55. doi: 10.1128/JCM.39.12.4247-4255.2001.
2
Recurrent bacteremia and multifocal lower limb cellulitis due to Helicobacter-like organisms in a patient with X-linked hypogammaglobulinemia.一名患有X连锁低丙种球蛋白血症的患者因类幽门螺杆菌感染出现复发性菌血症和多灶性下肢蜂窝织炎。
Clin Infect Dis. 2001 Nov 15;33(10):E116-8. doi: 10.1086/323405. Epub 2001 Oct 5.
3
"Helicobacter rappini" isolates from 2 homosexual men.从两名男同性恋者身上分离出的“拉平尼螺杆菌”菌株。
Clin Infect Dis. 2001 Jul 1;33(1):e8-11. doi: 10.1086/320881. Epub 2001 May 23.
4
Identification and detection of Stenotrophomonas maltophilia by rRNA-directed PCR.通过rRNA导向的聚合酶链反应鉴定和检测嗜麦芽窄食单胞菌
J Clin Microbiol. 2000 Dec;38(12):4305-9. doi: 10.1128/JCM.38.12.4305-4309.2000.
5
Rapid detection of HSV from cytologic specimens collected into ThinPrep fixative.从收集到ThinPrep固定剂中的细胞学标本中快速检测单纯疱疹病毒。
Acta Cytol. 1999 Nov-Dec;43(6):1034-8. doi: 10.1159/000331350.
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Analysis of mRNA quality in freshly prepared and archival Papanicolaou samples.
Acta Cytol. 1999 Sep-Oct;43(5):831-6. doi: 10.1159/000331298.
7
Comparison of culture and PCR for detection of Burkholderia cepacia in sputum samples of patients with cystic fibrosis.囊性纤维化患者痰液样本中检测洋葱伯克霍尔德菌的培养法与聚合酶链反应法比较
J Clin Microbiol. 1998 Jun;36(6):1642-5. doi: 10.1128/JCM.36.6.1642-1645.1998.
8
Bacteremia caused by Helicobacter cinaedi in an AIDS patients.一名艾滋病患者感染嗜肺埃希杆菌所致的菌血症。
J Formos Med Assoc. 1997 Jul;96(7):558-60.
9
Recurrent Helicobacter cinaedi cellulitis and bacteraemia in a patient with HIV Infection.一名HIV感染患者复发性西奈埃希菌蜂窝织炎和菌血症
Int J STD AIDS. 1997 Jan;8(1):59-60. doi: 10.1258/0956462971918634.
10
Polymerase chain reaction for the detection of Pseudomonas aeruginosa, Stenotrophomonas maltophilia and Burkholderia cepacia in sputum of patients with cystic fibrosis.聚合酶链反应检测囊性纤维化患者痰液中的铜绿假单胞菌、嗜麦芽窄食单胞菌和洋葱伯克霍尔德菌。
Mol Cell Probes. 1996 Dec;10(6):397-403. doi: 10.1006/mcpr.1996.0055.

通过对革兰氏染色材料进行直接基因分型分析，在一名重症人类免疫缺陷病毒感染患者中鉴定出百日咳博德特氏菌，并利用独特的recA基因限制性酶切位点与霍氏博德特氏菌进行区分。

Identification of Bordetella pertussis in a critically ill human immunodeficiency virus-infected patient by direct genotypical analysis of Gram-stained material and discrimination from B. holmesii by using a unique recA gene restriction enzyme site.

作者信息

Vielemeyer Ole, Crouch Jill Y, Edberg Stephen C, Howe John G

机构信息

Department of Laboratory Medicine, Yale University School of Medicine, New Haven, Connecticut 06520-8035, USA.

出版信息

J Clin Microbiol. 2004 Feb;42(2):847-9. doi: 10.1128/JCM.42.2.847-849.2004.

DOI:10.1128/JCM.42.2.847-849.2004
PMID:14766870
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC344463/
Abstract

Bordetella pertussis was diagnosed in a human immunodeficiency virus-infected patient by a newly developed method in which bacterial DNA is amplified directly from sputum Gram-stained slides. The validation of the method is described along with an additional new PCR-based assay that can distinguish between B. pertussis and Bordetella holmesii.

摘要

通过一种新开发的方法，即直接从痰革兰氏染色玻片上扩增细菌DNA，在一名感染人类免疫缺陷病毒的患者中诊断出百日咳博德特氏菌。文中描述了该方法的验证过程以及另一种基于聚合酶链反应的新检测方法，该方法可以区分百日咳博德特氏菌和霍氏博德特氏菌。