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新型双重实时 PCR 检测法可检测加拿大安大略省类百日咳症状患者标本中的博德特氏霍氏菌。

Novel duplex real-time PCR assay detects Bordetella holmesii in specimens from patients with Pertussis-like symptoms in Ontario, Canada.

机构信息

Ontario Agency for Health Protection and Promotion, Toronto, Ontario M9P 3T1, Canada.

出版信息

J Clin Microbiol. 2010 Apr;48(4):1435-7. doi: 10.1128/JCM.02417-09. Epub 2010 Feb 24.

DOI:10.1128/JCM.02417-09
PMID:20181919
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2849596/
Abstract

Bordetella holmesii is a human pathogen found mainly in immunocompromised patients. A specific real-time PCR assay was developed and successfully used to identify specimens from which B. holmesii was misidentified as Bordetella pertussis and to establish the prevalence of B. holmesii in Ontario patients with pertussis-like symptoms.

摘要

支气管败血博德特氏菌是一种主要存在于免疫功能低下患者中的人类病原体。本研究开发了一种特定的实时 PCR 检测方法，成功地用于鉴定将支气管败血博德特氏菌误鉴定为百日咳博德特氏菌的标本，并确定了安大略省具有类百日咳症状患者中支气管败血博德特氏菌的流行率。

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1
Novel duplex real-time PCR assay detects Bordetella holmesii in specimens from patients with Pertussis-like symptoms in Ontario, Canada.新型双重实时 PCR 检测法可检测加拿大安大略省类百日咳症状患者标本中的博德特氏霍氏菌。
J Clin Microbiol. 2010 Apr;48(4):1435-7. doi: 10.1128/JCM.02417-09. Epub 2010 Feb 24.
2
Real-time PCR assay targeting IS481 of Bordetella pertussis and molecular basis for detecting Bordetella holmesii.靶向百日咳博德特氏菌IS481的实时聚合酶链反应检测及检测霍氏博德特氏菌的分子基础
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本文引用的文献

1
Use of Bordetella pertussis BP3385 to establish a cutoff value for an IS481-targeted real-time PCR assay.使用百日咳博德特氏菌BP3385为针对IS481的实时PCR检测建立临界值。
J Clin Microbiol. 2008 Nov;46(11):3798-9. doi: 10.1128/JCM.01551-08. Epub 2008 Sep 10.
2
Community-acquired pneumonia due to Bordetella holmesii in a patient with frequently relapsing nephrotic syndrome.一名频繁复发肾病综合征患者感染霍氏博德特菌所致的社区获得性肺炎
J Infect. 2007 Apr;54(4):e203-5. doi: 10.1016/j.jinf.2006.11.004. Epub 2006 Dec 14.
3
Prevalence and sequence variants of IS481 in Bordetella bronchiseptica: implications for IS481-based detection of Bordetella pertussis.支气管败血波氏杆菌中IS481的流行情况和序列变异：对基于IS481的百日咳博德特氏菌检测的影响
J Clin Microbiol. 2006 Dec;44(12):4577-83. doi: 10.1128/JCM.01295-06. Epub 2006 Oct 25.
4
Bordetella holmesii DNA is not detected in nasopharyngeal swabs from Finnish and Dutch patients with suspected pertussis.在芬兰和荷兰疑似百日咳患者的鼻咽拭子中未检测到霍氏博德特氏菌DNA。
J Med Microbiol. 2006 Aug;55(Pt 8):1043-1051. doi: 10.1099/jmm.0.46331-0.
5
Bordetella holmesii bacteremia: a newly recognized clinical entity among asplenic patients.霍氏鲍特菌血症：无脾患者中一种新认识的临床实体。
Clin Infect Dis. 2004 Mar 15;38(6):799-804. doi: 10.1086/381888. Epub 2004 Mar 1.
6
Identification of Bordetella pertussis in a critically ill human immunodeficiency virus-infected patient by direct genotypical analysis of Gram-stained material and discrimination from B. holmesii by using a unique recA gene restriction enzyme site.通过对革兰氏染色材料进行直接基因分型分析，在一名重症人类免疫缺陷病毒感染患者中鉴定出百日咳博德特氏菌，并利用独特的recA基因限制性酶切位点与霍氏博德特氏菌进行区分。
J Clin Microbiol. 2004 Feb;42(2):847-9. doi: 10.1128/JCM.42.2.847-849.2004.
7
Multiplex LightCycler PCR assay for detection and differentiation of Bordetella pertussis and Bordetella parapertussis in nasopharyngeal specimens.用于检测和区分鼻咽标本中百日咳博德特氏菌和副百日咳博德特氏菌的多重LightCycler PCR检测法。
J Clin Microbiol. 2002 Jan;40(1):96-100. doi: 10.1128/JCM.40.1.96-100.2002.
8
Evaluation of a real-time PCR assay for detection of Bordetella pertussis and B. parapertussis in clinical samples.
J Med Microbiol. 2001 May;50(5):436-440. doi: 10.1099/0022-1317-50-5-436.
9
Real-time PCR assay targeting IS481 of Bordetella pertussis and molecular basis for detecting Bordetella holmesii.靶向百日咳博德特氏菌IS481的实时聚合酶链反应检测及检测霍氏博德特氏菌的分子基础
J Clin Microbiol. 2001 May;39(5):1963-6. doi: 10.1128/JCM.39.5.1963-1966.2001.
10
Recovery of Bordetella holmesii from patients with pertussis-like symptoms: use of pulsed-field gel electrophoresis to characterize circulating strains.从有百日咳样症状的患者中分离出霍氏博德特菌：使用脉冲场凝胶电泳对流行菌株进行特征分析。
J Clin Microbiol. 2000 Jun;38(6):2330-3. doi: 10.1128/JCM.38.6.2330-2333.2000.