新型双重实时 PCR 检测法可检测加拿大安大略省类百日咳症状患者标本中的博德特氏霍氏菌。
Novel duplex real-time PCR assay detects Bordetella holmesii in specimens from patients with Pertussis-like symptoms in Ontario, Canada.
机构信息
Ontario Agency for Health Protection and Promotion, Toronto, Ontario M9P 3T1, Canada.
出版信息
J Clin Microbiol. 2010 Apr;48(4):1435-7. doi: 10.1128/JCM.02417-09. Epub 2010 Feb 24.
Abstract
Bordetella holmesii is a human pathogen found mainly in immunocompromised patients. A specific real-time PCR assay was developed and successfully used to identify specimens from which B. holmesii was misidentified as Bordetella pertussis and to establish the prevalence of B. holmesii in Ontario patients with pertussis-like symptoms.
摘要
支气管败血博德特氏菌是一种主要存在于免疫功能低下患者中的人类病原体。本研究开发了一种特定的实时 PCR 检测方法，成功地用于鉴定将支气管败血博德特氏菌误鉴定为百日咳博德特氏菌的标本，并确定了安大略省具有类百日咳症状患者中支气管败血博德特氏菌的流行率。
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本文引用的文献
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Use of Bordetella pertussis BP3385 to establish a cutoff value for an IS481-targeted real-time PCR assay.使用百日咳博德特氏菌BP3385为针对IS481的实时PCR检测建立临界值。
J Clin Microbiol. 2008 Nov;46(11):3798-9. doi: 10.1128/JCM.01551-08. Epub 2008 Sep 10.
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Community-acquired pneumonia due to Bordetella holmesii in a patient with frequently relapsing nephrotic syndrome.一名频繁复发肾病综合征患者感染霍氏博德特菌所致的社区获得性肺炎
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Prevalence and sequence variants of IS481 in Bordetella bronchiseptica: implications for IS481-based detection of Bordetella pertussis.支气管败血波氏杆菌中IS481的流行情况和序列变异：对基于IS481的百日咳博德特氏菌检测的影响
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Bordetella holmesii DNA is not detected in nasopharyngeal swabs from Finnish and Dutch patients with suspected pertussis.在芬兰和荷兰疑似百日咳患者的鼻咽拭子中未检测到霍氏博德特氏菌DNA。
J Med Microbiol. 2006 Aug;55(Pt 8):1043-1051. doi: 10.1099/jmm.0.46331-0.
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