Martini Helena, Detemmerman Liselot, Soetens Oriane, Yusuf Erlangga, Piérard Denis
Vrije Universiteit Brussel (VUB), Universitair Ziekenhuis Brussel (UZ Brussel), Department of Microbiology and Infection Control and Belgian National Reference Centre for Bordetella Pertussis, Brussels, Belgium.
PLoS One. 2017 Apr 12;12(4):e0175587. doi: 10.1371/journal.pone.0175587. eCollection 2017.
The incidence of whooping cough, a contagious respiratory disease caused by Bordetella pertussis, is on the rise despite existing vaccination programmes. Similar, though usually milder, respiratory symptoms may be caused by other members of the Bordetella genus: B. parapertussis, B. holmesii, and B. bronchiseptica. Pertussis diagnosis is mostly done using PCR, but the use of multiple targets is necessary in order to differentiate the different Bordetella spp. with sufficient sensitivity and specificity. In this study we evaluate a multiplex PCR assay for the differentiation of B. pertussis from other Bordetella spp., using the targets IS481, IS1001, IS1002, and recA. Moreover, we retrospectively explore the epidemiology of Bordetella spp. infections in Belgium, using the aforementioned assay over a three-year period, from 2013 until 2015.
尽管已有疫苗接种计划，但由百日咳博德特氏菌引起的传染性呼吸道疾病百日咳的发病率仍在上升。百日咳博德特氏菌属的其他成员，如副百日咳博德特氏菌、霍氏博德特氏菌和支气管败血博德特氏菌，也可能引起类似但通常较轻的呼吸道症状。百日咳的诊断大多采用聚合酶链反应（PCR），但为了以足够的敏感性和特异性区分不同的博德特氏菌属，需要使用多个靶点。在本研究中，我们评估了一种多重PCR检测方法，利用IS481、IS1001、IS1002和recA靶点，从其他博德特氏菌属中区分出百日咳博德特氏菌。此外，我们回顾性地探讨了2013年至2015年三年间比利时博德特氏菌属感染的流行病学情况，采用了上述检测方法。
