Collins B H, Rosato E, Kyriacou C P
Department of Genetics, University of Leicester, Leicester LE1 7RH, United Kingdom.
Proc Natl Acad Sci U S A. 2004 Feb 17;101(7):1945-50. doi: 10.1073/pnas.0308240100. Epub 2004 Feb 6.
Drosophila melanogaster locomotor activity responds to different seasonal conditions by thermosensitive regulation of splicing of a 3' intron in the period mRNA transcript. Here we demonstrate that the control of locomotor patterns by this mechanism is primarily light-dependent at low temperatures. At warmer temperatures, when it is vitally important for the fly to avoid midday desiccation, more stringent regulation of splicing is observed, requiring the light input received through the visual system during the day and the circadian clock at night. During the course of this study, we observed that a mutation in the no-receptor-potential-A(P41) (norpA(P41)) gene, which encodes phospholipase-C, generated an extremely high level of 3' splicing. This cannot be explained simply by the mutation's effect on the visual pathway and suggests that norpA(P41) is directly involved in thermosensitivity.
黑腹果蝇的运动活动通过对周期基因（period）mRNA转录本中一个3'内含子的剪接进行温度敏感调节，来响应不同的季节条件。在此我们证明，在低温下，通过这种机制对运动模式的控制主要依赖于光。在较温暖的温度下，当果蝇避免中午干燥至关重要时，会观察到对剪接更严格的调节，这需要白天通过视觉系统接收的光输入以及夜间的生物钟。在这项研究过程中，我们观察到编码磷脂酶C的无受体电位A（P41）（norpA（P41））基因突变产生了极高水平的3'剪接。这不能简单地用该突变对视觉通路的影响来解释，表明norpA（P41）直接参与温度敏感性。
