Shcherbakova V A, Chuvil'skaia N A, Golovchenko N P, Suzina N E, Lysenko A M, Laurinavichus K S, Akimenko V K
Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr. Nauki 5, Pushchino, Moscow Oblast, 142290 Russia.
Mikrobiologiia. 2003 Nov-Dec;72(6):752-8.
Three strains of Clostridium sp., 14 (VKM B-2201), 42 (VKM B-2202), and 21 (VKM B-2279), two methanogens, Methanobacterium formicicum MH (VKM B-2198) and Methanosarcina mazei MM (VKM B-2199), and one sulfate-reducing bacterium, Desulfovibrio sp. SR1 (VKM B-2200), were isolated in pure cultures from an anaerobic microbial community capable of degrading p-toluene sulfonate. Strain 14 was able to degrade p-toluene sulfonate in the presence of yeast extract and bactotryptone and, like strain 42, to utilize p-toluene sulfonate as the sole sulfur source with the production of toluene. p-Toluene sulfonate stimulated the growth of Ms. mazei MM on acetate. The sulfate-reducing strain Desulfovibrio sp. SR1 utilized p-toluene sulfonate as an electron acceptor. The putative scheme of p-toluene sulfonate degradation by the anaerobic microbial community is discussed.
从一个能够降解对甲苯磺酸盐的厌氧微生物群落中分离出了三株梭菌属菌株，分别是14号菌株（VKM B - 2201）、42号菌株（VKM B - 2202）和21号菌株（VKM B - 2279），两株产甲烷菌，甲酸甲烷杆菌MH（VKM B - 2198）和马氏甲烷八叠球菌MM（VKM B - 2199），以及一株硫酸盐还原菌，脱硫弧菌属SR1（VKM B - 2200）。14号菌株能够在酵母提取物和胰蛋白胨存在的情况下降解对甲苯磺酸盐，并且与42号菌株一样，能够将对甲苯磺酸盐作为唯一硫源利用并产生甲苯。对甲苯磺酸盐刺激了马氏甲烷八叠球菌MM在乙酸盐上的生长。硫酸盐还原菌株脱硫弧菌属SR1将对甲苯磺酸盐用作电子受体。文中讨论了厌氧微生物群落降解对甲苯磺酸盐的推测方案。
