Lundqvist C A, Taubman M A, Stoufi E D, Liu X P, Smith D J
Department of Immunology, Forsyth Dental Center, Boston, MA 02115.
Reg Immunol. 1992 Jul-Aug;4(4):255-61.
Gingival mononuclear cells from patients with adult periodontitis were cultured to determine the potential for IgG production. All samples (N = 27) showed IgG synthesis. Some samples demonstrated IgG synthetic activity over the entire period in culture, often with maximum synthesis after 8 days. Other samples showed IgG synthesis during the first half of the culture period and then little detectable production for the remainder. Cells were either untreated or treated with one of several different known mitogens during the culture period. Total IgG synthesis by peripheral blood lymphocytes was enhanced in the presence of pokeweed mitogen, E. coli lipopolysaccharide and killed. A. actinomycetemcomitans. In contrast, IgG synthesis by gingival cells in the presence of these same additives was significantly reduced when compared to gingival cell synthesis in the absence of mitogens; suggesting the presence of suppression in this system. These differences in responsiveness may be attributable to the unique combination of T cells found in the gingival tissues of patients with periodontal diseases. The patterns of IgG synthesis by gingival cells were different from those of peripheral blood cells from the same patient. This finding verified the distinctiveness of local immunoregulatory mechanisms in periodontal disease tissue from those found systemically.
培养成人牙周炎患者的牙龈单核细胞，以确定其产生IgG的潜力。所有样本（N = 27）均显示有IgG合成。一些样本在整个培养期间都表现出IgG合成活性，通常在8天后合成量达到最大。其他样本在培养期的前半段显示有IgG合成，而在其余时间则几乎检测不到产量。细胞在培养期间要么未处理，要么用几种不同的已知促细胞分裂剂之一进行处理。在美洲商陆促细胞分裂剂、大肠杆菌脂多糖和灭活的伴放线放线杆菌存在的情况下，外周血淋巴细胞的总IgG合成增强。相比之下，与不存在促细胞分裂剂时牙龈细胞的合成相比，在这些相同添加剂存在的情况下牙龈细胞的IgG合成显著减少；这表明该系统中存在抑制作用。这些反应性差异可能归因于牙周病患者牙龈组织中发现的T细胞的独特组合。牙龈细胞的IgG合成模式与同一患者外周血细胞的模式不同。这一发现证实了牙周病组织中局部免疫调节机制与全身免疫调节机制的不同。
