人淋巴因子激活的杀伤细胞抑制商陆有丝分裂原诱导的免疫球蛋白合成。
Human lymphokine-activated killer cells suppress pokeweed mitogen-induced immunoglobulin synthesis.
作者信息
Suzuki H, Yamashita N, Maruyama M, Yoshikawa T, Yano S
机构信息
First Department of Internal Medicine, Toyama Medical and Pharmaceutical University, School of Medicine, Japan.
出版信息
Clin Exp Immunol. 1989 Dec;78(3):406-11.
The effect of human lymphokine-activated killer (LAK) cells on pokeweed mitogen (PWM) induced immunoglobulin synthesis by autologous peripheral blood mononuclear cells (PBMC) was studied. LAK cells induced by the in vitro culture with recombinant human interleukin-2 (IL-2) lysed PWM-activated autologous T cells and B cells, but did not lyse unstimulated lymphocytes. These effector cells which are capable of killing lymphoblasts were shown to express either CD16 surface markers. When CD8(+)-and CD16(+)-enriched cells isolated from the culture with IL-2 were added to cultures containing autologous PBMC and PWM, marked suppression of the IgG production was observed. In contrast, the control CD8(+)-and CD16(+)-enriched cells isolated from the culture without IL-2 showed a weak suppressive effect on PWM-induced IgG synthesis. These results suggest that LAK cells suppress immunoglobulin synthesis by the cytotoxic elimination of activated T cells and B cells.
研究了人淋巴因子激活的杀伤（LAK）细胞对美洲商陆有丝分裂原（PWM）诱导的自体外周血单个核细胞（PBMC）免疫球蛋白合成的影响。用重组人白细胞介素-2（IL-2）体外培养诱导的LAK细胞可裂解PWM激活的自体T细胞和B细胞，但不能裂解未受刺激的淋巴细胞。这些能够杀伤淋巴母细胞的效应细胞显示表达CD16表面标志物。当将从含IL-2的培养物中分离出的富含CD8(+)和CD16(+)的细胞加入到含有自体PBMC和PWM的培养物中时，观察到IgG产生受到明显抑制。相比之下，从不含IL-2的培养物中分离出的对照富含CD8(+)和CD16(+)的细胞对PWM诱导的IgG合成显示出较弱的抑制作用。这些结果表明，LAK细胞通过细胞毒性消除活化的T细胞和B细胞来抑制免疫球蛋白合成。
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