Pautard B, Rémy J J, Charreire J
Clin Exp Immunol. 1986 Nov;66(2):435-42.
Lymphocytes from freshly diagnosed untreated GD patients can be induced to produce in-vitro IgG, and more precisely TSI in the presence of PWM, a T cell dependent polyclonal activator of B cells. TSI were measured from days 8 to 41 of culture, using a functional cAMP production assay, after the deposition of culture supernatants on HTEC. Spontaneous or PWM induced IgG synthesis was optimal on day 28 of culture for GD MNC, with amounts of IgG in PWM stimulated GD culture supernatants twice those found in unstimulated GD MNC. Moreover only PWM stimulated GD MNC produce IgG which induce cAMP production by cultured HTEC, with a significant correlation between in-vitro IgG and cAMP levels.
来自新诊断未治疗的格雷夫斯病（GD）患者的淋巴细胞在PWM（一种T细胞依赖性B细胞多克隆激活剂）存在的情况下可被诱导在体外产生IgG，更确切地说是TSI。在培养上清液沉积在人甲状腺上皮细胞（HTEC）上后，使用功能性cAMP产生测定法，在培养的第8天至41天测量TSI。对于GD单个核细胞（MNC），培养第28天时自发或PWM诱导的IgG合成最佳，PWM刺激的GD培养上清液中的IgG量是未刺激的GD MNC中发现量的两倍。此外，只有PWM刺激的GD MNC产生可诱导培养的HTEC产生cAMP的IgG，体外IgG与cAMP水平之间存在显著相关性。
