Asayama S, Mori T, Nagaoka S, Kawakami H
Department of Applied Chemistry, Tokyo Metropolitan University. Minami-Osawa, Hachioji, Tokyo 192-0397, Japan.
J Biomater Sci Polym Ed. 2003;14(11):1169-79. doi: 10.1163/156856203322553419.
Superoxide dismutase (SOD), which catalyzes the reduction of O2*- to H2O2, is the key enzyme for the protection of oxidative stress. Here we have chemically modified manganese (Mn) porphyrins with biomolecules for new functional antioxidants. The Mn-porphyrins were conjugated with the following biochemical functional molecules: (1) catalase, to catalyze reduction of H2O2 to H2O. The resulting conjugate showed dual functions of SOD and catalase; (2) a carbohydrate, to facilitate receptor binding and, hence, active targeting. The resulting conjugate showed both SOD activity and carbohydrate recognition. These results suggest that the antioxidants promise the application to biomedical fields.
超氧化物歧化酶（SOD）可催化将超氧阴离子还原为过氧化氢，是保护细胞免受氧化应激的关键酶。在此，我们用生物分子对锰（Mn）卟啉进行了化学修饰，以制备新型功能性抗氧化剂。将锰卟啉与以下生化功能分子共轭：（1）过氧化氢酶，用于催化将过氧化氢还原为水。所得共轭物表现出超氧化物歧化酶和过氧化氢酶的双重功能；（2）一种碳水化合物，用于促进受体结合，从而实现主动靶向。所得共轭物兼具超氧化物歧化酶活性和碳水化合物识别能力。这些结果表明，这些抗氧化剂有望应用于生物医学领域。
