Umakoshi Hiroshi, Morimoto Kengo, Ohama Yuji, Nagami Hideto, Shimanouchi Toshinori, Kuboi Ryoichi
Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan.
Langmuir. 2008 May 6;24(9):4451-5. doi: 10.1021/la800174n. Epub 2008 Mar 27.
An antioxidative liposome catalysis that mimics both superoxide dismutase (SOD) and peroxidase (POD) activities has been developed by using the liposomes modified with lipophilic Mn-(5,10,15,20-tetrakis[1-hexadecylpyridium-4-yl]-21H,23H-porphyrin) (Mn-HPyP). The SOD- and POD-like activities of the Mn-HPyP-modified liposome were first investigated by varying the type of phospholipid, such as 1,2-distearyl-sn-glycero-3-phosphocholine (DSPC), 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), and 1,2-dilauroyl-sn-glycero-3-phosphocholine (DLPC). Higher SOD-like activity was obtained in the case of DLPC and DMPC liposomes, in which the ligands were well-dispersed on the membrane in the liquid crystalline phase. The POD-like activity was maximal in the case of DMPC liposome, in which the Mn-HPyP complex was appropriately clustered on the membrane in the gel phase. On the basis of the above results, the co-induction of the SOD and POD activities to eliminate the superoxide and also hydrogen peroxide as a one-pot reaction was finally performed by using the Mn-HPyP-modified DMPC liposome, resulting in an increase in the efficiency of the elimination of both superoxide and hydrogen peroxide.
通过使用用亲脂性锰（5,10,15,20-四[1-十六烷基吡啶-4-基]-21H,23H-卟啉）（Mn-HPyP）修饰的脂质体，开发了一种模拟超氧化物歧化酶（SOD）和过氧化物酶（POD）活性的抗氧化脂质体催化体系。首先通过改变磷脂类型，如1,2-二硬脂酰-sn-甘油-3-磷酸胆碱（DSPC）、1,2-二肉豆蔻酰-sn-甘油-3-磷酸胆碱（DMPC）、1,2-二棕榈酰-sn-甘油-3-磷酸胆碱（DPPC）和1,2-二月桂酰-sn-甘油-3-磷酸胆碱（DLPC），研究了Mn-HPyP修饰脂质体的类SOD和类POD活性。在DLPC和DMPC脂质体中获得了较高的类SOD活性，其中配体在液晶相中很好地分散在膜上。类POD活性在DMPC脂质体中最大，其中Mn-HPyP配合物在凝胶相中适当地聚集在膜上。基于上述结果，最终通过使用Mn-HPyP修饰的DMPC脂质体进行SOD和POD活性的共诱导，以一锅反应消除超氧化物和过氧化氢，从而提高了超氧化物和过氧化氢的消除效率。
