Terao Ken, Nagasawa Naotsugu, Nishida Hirokazu, Furusawa Kazuya, Mori Yasutaka, Yoshii Fumio, Dobashi Toshiaki
Department of Biological and Chemical Engineering, Faculty of Engineering, Gunma University, Kiryu, Gunma 376-8515, Japan.
J Biomater Sci Polym Ed. 2003;14(11):1197-208. doi: 10.1163/156856203322553437.
In order to obtain a gelatin hydrogel crosslinked by a reagent-free method, gamma-ray and electron beam radiation was applied to porcine, bovine and fish gelatin gels and the products were characterized by measuring the gel fraction, the swelling ratio and the enzymatic degradability. On increasing the radiation dose, the gel fraction increased and both the swelling ratio and the enzymatic degradability decreased. The transition temperature from gel to sol of the hydrogel containing more than 5% mammal gelatins increased up to more than 90 degrees C when gamma-ray or electron beam were irradiated by more than 10 kGy. The results show that the degree of crosslinking of irradiated gelatin hydrogels increases with increasing irradiation dose and with decreasing concentration. It is suggested that the radiation crosslinking occurs around the physical crosslinking point or multiple helix structure of gelatin gel.
为了获得通过无试剂方法交联的明胶水凝胶，对猪、牛和鱼明胶凝胶施加了γ射线和电子束辐射，并通过测量凝胶分数、溶胀率和酶降解性对产物进行了表征。随着辐射剂量的增加，凝胶分数增加，溶胀率和酶降解性均降低。当γ射线或电子束以超过10 kGy的剂量照射时，含有超过5%哺乳动物明胶的水凝胶从凝胶到溶胶的转变温度升高至超过90℃。结果表明，辐照明胶水凝胶的交联度随着辐照剂量的增加和浓度的降低而增加。有人认为，辐射交联发生在明胶凝胶的物理交联点或多重螺旋结构周围。
