Tseng-Rogenski Stephanie S, Chang Tien-Hsien
Department of Molecular Genetics, The Ohio State University, Columbus, OH, USA.
Methods Mol Biol. 2004;257:93-102. doi: 10.1385/1-59259-750-5:093.
The evolutionarily conserved DExD/H-box proteins are essential for all RNA-related biological processes. They are thought to modulate the structure and function of specific RNAs and/or ribonucleoprotein particles by using their intrinsic RNA-dependent ATPase activities to achieve the desired conformational changes. A number of DExD/H-box proteins have been shown to unwind short RNA duplexes in vitro, a hallmark of the so-called RNA helicases or unwindases. However, some are unable to do so, perhaps because of requirements for cofactors. Here, we present a "solid-state" method that may allow investigators to overcome such problems.
进化上保守的DExD/H盒蛋白对于所有与RNA相关的生物学过程至关重要。人们认为它们通过利用其内在的RNA依赖性ATP酶活性来调节特定RNA和/或核糖核蛋白颗粒的结构和功能，以实现所需的构象变化。许多DExD/H盒蛋白已被证明在体外能解开短RNA双链体，这是所谓RNA解旋酶或解旋酶的一个标志。然而，有些蛋白无法做到这一点，可能是因为对辅助因子有要求。在这里，我们提出一种“固态”方法，该方法可能使研究人员克服此类问题。
