[人绒毛膜绒毛中的苯丙氨酸羟化酶样抗体]

[Phenylalanine hydroxylase-like antibody in human chorionic villi].

作者信息

Chestkov V V, Shepkina Iu V, Jennings I G, Cotton R G, Shishkin S S

出版信息

Biull Eksp Biol Med. 1992 Sep;114(9):308-10.

Abstract

An antigen similar by electrophoretic mobility to liver phenylalanine hydroxylase (PH) and cross-reacting with monoclonal antibody PH8 against liver PH was detected in extracts of soluble proteins in 6 from 23 samples of chorionic villi. An antigen with electrophoretic mobility corresponding to 40-41 kDa was detected in extracts of membrane proteins from these 23 samples by immunoblotting with monoclonal antibody PH8. Its molecular weight was similar to that of major chymotryptic peptide of human liver PH. The content of the antigen varied with samples and was less than 20 ng/mg of the extracted protein. Two-dimensional gel electrophoresis revealed only 1 spot of the antigen. The antigen did not react with monoclonal antibodies PH7 and PH9 epitopes of which were located in N-terminal fragment of liver PH. These data suggest that the antigen of membrane fraction could be a PH protein without N-terminal domain.

摘要

在23份绒毛膜绒毛样本中的6份样本的可溶性蛋白质提取物中，检测到一种抗原，其电泳迁移率与肝脏苯丙氨酸羟化酶（PH）相似，且能与抗肝脏PH的单克隆抗体PH8发生交叉反应。通过用单克隆抗体PH8进行免疫印迹，在这23份样本的膜蛋白提取物中检测到一种电泳迁移率对应于40 - 41 kDa的抗原。其分子量与人肝脏PH的主要胰凝乳蛋白酶肽段相似。该抗原的含量因样本而异，且低于每毫克提取蛋白20 ng。二维凝胶电泳仅显示出该抗原的1个斑点。该抗原不与单克隆抗体PH7和PH9反应，其表位位于肝脏PH的N端片段。这些数据表明，膜部分的抗原可能是一种没有N端结构域的PH蛋白。