Laptev A V, Chestkov V V
Ontogenez. 1990 Mar-Apr;21(2):138-44.
Content, subunit composition and activity of phenylalanine hydroxylase's (PH) (EC 1.14.16.1) in cytoplasmic and membrane proteins extract of embryonic liver on week 6-11 of pregnancy were studied. PH enzymatic and antigenic activities were detected starting from the week 6 of pregnancy. Liver cytoplasmic PH antigen content increased gradually during development while its enzymatic activity remained practically unchanged. Concomitantly, relative content of L-subunit increased. Content of liver membrane PH antigen was constant during development. Samples of liver with relatively low specific PH activity were characterized by high content of PH in cytoplasm and vice versa. Since PH activity in extracts prepared from mixture of these samples decreased, an unknown PH inhibitor must be present in cytoplasmic protein extracts with relatively low specific PH activity.
研究了妊娠6 - 11周胚胎肝脏细胞质和膜蛋白提取物中苯丙氨酸羟化酶（PH）（EC 1.14.16.1）的含量、亚基组成及活性。从妊娠第6周开始检测到PH的酶活性和抗原活性。肝脏细胞质PH抗原含量在发育过程中逐渐增加，而其酶活性基本保持不变。同时，L亚基的相对含量增加。肝脏膜PH抗原含量在发育过程中保持恒定。具有相对较低特异性PH活性的肝脏样品的特征是细胞质中PH含量高，反之亦然。由于从这些样品混合物制备的提取物中的PH活性降低，因此在具有相对较低特异性PH活性的细胞质蛋白提取物中必定存在一种未知的PH抑制剂。
