Laptev A V, Chestkov V V, Shchepkina Iu V, Shishkin S S
Ontogenez. 1988 Jul-Aug;19(4):385-9.
Phenylalanine hydroxylase (PH) activity was discovered in the liver of 7-12 week old human embryos. Embryonic and adult PHs were identical, as shown by immunoelectrophoresis. Unlike the adult liver PH, the PH content of the extract of cytoplasmic proteins of embryonic liver was reduced but the specific activity was increased more than by one order of magnitude. H (57,000 D) and L (55,000 D) subunits were detected by immunoblotting. The L subunit predominates in the extract of membrane proteins of embryonic liver. Hence, the major part of phenylalanine oxidizing activity in the embryonic liver is related to the enzyme immunochemically identical with the PH of adult liver but differing from it in some structural and functional properties.
在7至12周龄人类胚胎的肝脏中发现了苯丙氨酸羟化酶（PH）活性。免疫电泳显示，胚胎期和成年期的PH是相同的。与成年肝脏PH不同，胚胎肝脏细胞质蛋白提取物中的PH含量降低，但比活性增加了一个多数量级以上。通过免疫印迹检测到了H（57,000 D）和L（55,000 D）亚基。L亚基在胚胎肝脏膜蛋白提取物中占主导地位。因此，胚胎肝脏中苯丙氨酸氧化活性的主要部分与成年肝脏PH免疫化学相同，但在某些结构和功能特性上与之不同的酶有关。
