Chestkov V V, Laptev A V, Shchepkina Iu V
Vopr Med Khim. 1988 May-Jun;34(3):113-6.
Phenylalanine hydroxylase was found in extracts from autoptates of human liver, kidney, myocardium, small intestine, lung and spleen. In all the tissues studied, except of spleen, the antigen was detected by immunoelectrophoresis with monospecific antiserum against human liver phenylalanine hydroxylase. As shown by immunoblotting carried out after electrophoresis under denaturating conditions, the antigen, observed in various tissues, exhibited similar electrophoretic mobility which was very close to that of the enzyme purified from human liver tissue. Molecular mass of revealed antigen was estimated 55-57 kDa. Coincidence of immunochemical and chemical properties of the protein suggested that the antigen, detected in the tissue extracts, was a product of phenylalanine hydroxylase gene expression. The antigen concentration did not correlate with the content of albumin in tissue extracts, thus demonstrating that the revealed antigen did not occur in these preparations with blood contaminations. Content of the antigen in the tissue extracts studied was (ug per g): liver - 1500-1900, kidney - 300-575, brain - 20-40, myocardium - 85-105, lung - 40-125, small intestine - 45-70, spleen - 0-12.
在取自人类肝脏、肾脏、心肌、小肠、肺和脾脏的尸检组织提取物中发现了苯丙氨酸羟化酶。在所有研究的组织中，除了脾脏外，用针对人肝脏苯丙氨酸羟化酶的单特异性抗血清通过免疫电泳检测到了该抗原。如在变性条件下电泳后进行的免疫印迹所示，在各种组织中观察到的抗原表现出相似的电泳迁移率，这与从人肝脏组织中纯化的酶非常接近。所揭示抗原的分子量估计为55 - 57 kDa。该蛋白质免疫化学和化学性质的一致性表明，在组织提取物中检测到的抗原是苯丙氨酸羟化酶基因表达的产物。抗原浓度与组织提取物中白蛋白的含量无关，从而表明在这些制剂中所揭示的抗原并非来自血液污染。所研究的组织提取物中抗原的含量（每克微克数）为：肝脏 - 1500 - 1900，肾脏 - 300 - 575，大脑 - 20 - 40，心肌 - 85 - 105，肺 - 40 - 125，小肠 - 45 - 70，脾脏 - 0 - 12。
