Takematsu H, Tagami H
Department of Dermatology, Tohoku University School of Medicine, Sendai, Japan.
Dermatology. 1992;185(4):246-50. doi: 10.1159/000247461.
The complement system is thought to play an important role in the recruitment of neutrophils within the epidermis. In the present study we examined whether or not complement activation in psoriatic lesional skin results in the deposition of terminal complement complexes within the epidermis by measuring levels of SC5b-9 in the plasma and horny tissues of psoriatic patients. The levels of SC5b-9 in psoriatic plasma were significantly higher than those of controls or those of patients with atopic dermatitis. However, when the levels of SC5b-9 in the psoriatic plasma were compared before and after successful treatment of psoriasis, a significant reduction was observed after treatment. Studies of total protein extracts from lesional skin showed that, while no SC5b-9 was detected in the noninflammatory horny tissues, there were high levels of SC5b-9 in lesional horny tissues of psoriasis. By immunofluorescence using a monoclonal antibody to the C5b-9 neoantigen, deposition of C5b-9 was observed only in the stratum corneum of psoriatic skin. Thus the results of the present study suggests that in psoriatic lesional skin, the complement system is activated and that this complement activation proceeds all the way to the terminal step, generating membrane attack complex.
补体系统被认为在表皮内中性粒细胞的募集过程中发挥重要作用。在本研究中，我们通过检测银屑病患者血浆和角质组织中SC5b-9的水平，来研究银屑病皮损皮肤中的补体激活是否会导致终末补体复合物在表皮内沉积。银屑病血浆中SC5b-9的水平显著高于对照组或特应性皮炎患者。然而，比较银屑病成功治疗前后血浆中SC5b-9的水平，治疗后观察到显著降低。对皮损皮肤总蛋白提取物的研究表明，在非炎症性角质组织中未检测到SC5b-9，而在银屑病皮损角质组织中有高水平的SC5b-9。通过使用针对C5b-9新抗原的单克隆抗体进行免疫荧光检测，仅在银屑病皮肤的角质层中观察到C5b-9的沉积。因此，本研究结果表明，在银屑病皮损皮肤中，补体系统被激活，并且这种补体激活一直进行到终末步骤，产生膜攻击复合物。
