Dangl J L, Ritter C, Gibbon M J, Mur L A, Wood J R, Goss S, Mansfield J, Taylor J D, Vivian A
Max-Delbrück-Laboratory, Köln, Germany.
Plant Cell. 1992 Nov;4(11):1359-69. doi: 10.1105/tpc.4.11.1359.
We showed that a bacterial avirulence (avr) gene function, avrPpiA1, from the pea pathogen Pseudomonas syringae pv pisi, is recognized by some, but not all, genotypes of Arabidopsis. Thus, an avr gene functionally defined on a crop species is also an avr gene on Arabidopsis. The activity of avrPpiA1 on a series of Arabidopsis genotypes is identical to that of the avrRpm1 gene from P.s. pv maculicola previously defined using Arabidopsis. The two avr genes are homologous and encode nearly identical predicted products. Moreover, this conserved avr function is also recognized by some bean and pea cultivars in what has been shown to be a gene-for-gene manner. We further demonstrated that the Arabidopsis disease resistance locus, RPM1, conditioning resistance to avrRpm1, also conditions resistance to bacterial strains carrying avrPpiA1. Therefore, bean, pea, and conceivably other crop species contain functional and potentially molecular homologs of RPM1.
我们发现，豌豆病原体丁香假单胞菌豌豆致病变种中的一个细菌无毒（avr）基因功能，即avrPpiA1，能被部分但并非所有拟南芥基因型识别。因此，在一种作物物种上功能定义的avr基因在拟南芥上也是一个avr基因。avrPpiA1在一系列拟南芥基因型上的活性与先前利用拟南芥定义的来自丁香假单胞菌番茄致病变种的avrRpm1基因的活性相同。这两个avr基因是同源的，编码几乎相同的预测产物。此外，这种保守的avr功能也被一些豆类和豌豆品种以基因对基因的方式识别。我们进一步证明，拟南芥抗病基因座RPM1，它决定对avrRpm1的抗性，也决定对携带avrPpiA1的细菌菌株的抗性。因此，豆类、豌豆以及可以想象的其他作物物种都含有RPM1的功能和潜在分子同源物。
