Leng J, Xu B D
Department of Pathology, Nanjing Medical college.
Zhonghua Bing Li Xue Za Zhi. 1992 Jun;21(3):160-3.
Intraperitoneal inoculation of duck hepatitis B virus in three different dosages (9 x 10(7), 1.8 x 10(8), 9 x 10(8) DHBV particles) into 3 to 21 day-old Chinese ducklings provided from a DHBV free flock produced a persistent infection up to 93.3% in 60 animals. The serum and liver specimens of these ducklings were examined by DNA dot blot hybridization on the 30th day after inoculation. The results showed that: (1) examination of viral DNA in liver was more sensitive and reliable than estimation of the DNA in serum for detecting DHBV infection in inoculated ducklings; (2) the liver DHBV DNA level did not coincide with the degree of liver hepatitis induced; (3) 21-day-old Chinese ducklings were also susceptible to DHBV infection, the infection rate of this group was 100% (10/10).
将三种不同剂量（9×10⁷、1.8×10⁸、9×10⁸个鸭乙型肝炎病毒颗粒）的鸭乙型肝炎病毒经腹腔接种到来自无鸭乙型肝炎病毒鸡群的3至21日龄中国雏鸭体内，在60只动物中产生了高达93.3%的持续感染。在接种后第30天，通过DNA斑点印迹杂交法对这些雏鸭的血清和肝脏标本进行检测。结果显示：（1）检测接种雏鸭体内的鸭乙型肝炎病毒感染时，检测肝脏中的病毒DNA比检测血清中的DNA更敏感、可靠；（2）肝脏鸭乙型肝炎病毒DNA水平与所诱发的肝炎程度不一致；（3）21日龄的中国雏鸭也易感染鸭乙型肝炎病毒，该组的感染率为100%（10/10）。
