Kim H M, Hirota S, Onoue H, Hirata T, Suzuki K, Ohno S, Kuroki T, Kitamura Y, Nomura S
Department of Pathology, Osaka University Medical School, Japan.
Brain Res Dev Brain Res. 1992 Dec 18;70(2):239-44. doi: 10.1016/0165-3806(92)90203-9.
The expression and localization of a novel protein kinase C delta (nPKC delta) mRNA were investigated using Northern blotting and in situ hybridization in the developmental process of mouse brain. In adult mice, nPKC delta was abundantly expressed in the thalamus, moderately in the pons and the cerebellum, but faintly in the cerebral cortex and the spinal cord. By in situ hybridization, the signals were observed specifically at the sensory and motor relay nuclei of the thalamus, the dorsal cochlear nuclei of the pons, and the molecular layer of the cerebellum. When developmental changes in the expression of nPKC delta gene were analyzed by in situ hybridization, it was not detectable in embryonic and neonatal brains, very weakly expressed in the thalamus in the first week, and highly expressed at two weeks of age. These results suggest that the gene expression of nPKC delta is strictly controlled by both the cell type and the developmental process.
利用Northern印迹法和原位杂交技术，对小鼠脑发育过程中一种新型蛋白激酶Cδ（nPKCδ）mRNA的表达和定位进行了研究。在成年小鼠中，nPKCδ在丘脑中大量表达，在脑桥和小脑中适度表达，但在大脑皮层和脊髓中表达较弱。通过原位杂交，在丘脑的感觉和运动中继核、脑桥的背侧耳蜗核以及小脑的分子层中特异性地观察到信号。当通过原位杂交分析nPKCδ基因表达的发育变化时，在胚胎和新生脑中未检测到，在第一周丘脑中表达非常弱，在两周龄时高表达。这些结果表明，nPKCδ的基因表达受到细胞类型和发育过程的严格控制。
