Goochee C F
Department of Chemical Engineering, Stanford University, CA 94305-5025.
Dev Biol Stand. 1992;76:95-104.
The oligosaccharide structures of glycoproteins can have a profound effect on properties critical to the development of glycoprotein products for human therapeutic or diagnostic use, including clearance rate, antigenicity, specific activity, solubility, resistance to thermal inactivation, and resistance to protease attack. Therefore, it is important to understand how bioprocess factors influence oligosaccharide structure. In this presentation, I will summarize literature data concerning potential effects of bioprocess factors on glycoprotein oligosaccharide biosynthesis. These data are drawn from two recent detailed reviews published by our laboratory describing the effect of cell culture conditions on N-linked glycosylation (10) and the effects of other bioprocess factors on both N-linked and O-linked glycosylation (11).
糖蛋白的寡糖结构可对用于人类治疗或诊断用途的糖蛋白产品开发的关键特性产生深远影响，这些特性包括清除率、抗原性、比活性、溶解度、耐热失活性以及抗蛋白酶攻击能力。因此，了解生物工艺因素如何影响寡糖结构很重要。在本报告中，我将总结有关生物工艺因素对糖蛋白寡糖生物合成潜在影响的文献数据。这些数据来自我们实验室最近发表的两篇详细综述，一篇描述细胞培养条件对N-连接糖基化的影响(10)，另一篇描述其他生物工艺因素对N-连接和O-连接糖基化的影响(11)。
