Jäger S, Demleitner G, Götz F
Mikrobielle Genetik, Universität Tübingen, FRG.
FEMS Microbiol Lett. 1992 Dec 15;100(1-3):249-54. doi: 10.1111/j.1574-6968.1992.tb14048.x.
In this study the putative catalytic triad Ser-His-Asp of the Staphylococcus hyicus ssp. hyicus lipase was investigated. Putative catalytic sites determined by homology comparisons of three staphylococcal and other non-staphylococcal lipases were altered by site-directed mutagenesis. Since the mutations did not influence the secretion of the lipase, the decrease in lipase activity of the mutants strongly supports the proposed involvement of Ser369 and His600 in catalysis. Asp559 is postulated to be the third amino acid of the triad.
在本研究中，对猪葡萄球菌亚种猪葡萄球菌脂肪酶假定的催化三联体Ser-His-Asp进行了研究。通过对三种葡萄球菌脂肪酶和其他非葡萄球菌脂肪酶进行同源性比较确定的假定催化位点，经定点诱变发生了改变。由于这些突变不影响脂肪酶的分泌，突变体脂肪酶活性的降低有力地支持了Ser369和His600参与催化作用的观点。推测Asp559是该三联体的第三个氨基酸。
