Frosch M, Meyer T F
Institut für Medizinische Mikrobiologie, Medizinische Hochschule Hannover, FRG.
FEMS Microbiol Lett. 1992 Dec 15;100(1-3):345-9. doi: 10.1111/j.1574-6968.1992.tb14062.x.
The horizontal flow of genetic material between microbes utilizes three principal routes: conjugation, transduction and transformation. While the significance in nature of the first two pathways is generally accepted, the in vivo role of transformation remains uncertain, despite the early observations by Griffith in 1928 on the transformation of streptococci from an avirulent to a virulent state [1]. Recently, circumstantial evidence was collected suggesting a role for transformation-mediated horizontal exchange in the modulation of virulence determinants of pathogenic Neisseriae and the variation of surface structures. In order to further assess the significance of transformation-mediated exchange we performed simple co-cultivation experiments of different Neisseria strains. We observed an efficient intra- and interspecies transfer of essential virulence determinants; the process was sensitive to the presence of DNaseI in the culture and was blocked in transformation-deficient recipients.
接合、转导和转化。虽然前两种途径在自然界中的重要性已被普遍接受，但尽管1928年格里菲斯早期观察到链球菌从无毒状态转变为有毒状态[1]，转化在体内的作用仍不确定。最近，收集到的间接证据表明，转化介导的水平交换在致病性奈瑟菌毒力决定因素的调节和表面结构的变异中发挥作用。为了进一步评估转化介导的交换的重要性，我们对不同的奈瑟菌菌株进行了简单的共培养实验。我们观察到了必需毒力决定因素在种内和种间的有效转移；该过程对培养物中DNaseI的存在敏感，并且在缺乏转化能力的受体中被阻断。
