Meyer Thomas, Buder Susanne
Department of Dermatology, Venerology and Allergology, St. Josef Hospital, Ruhr-University, Bochum 44791, Germany.
German Consiliary Laboratory for Gonococci, Department of Dermatology and Venerology, Vivantes Hospital Berlin, Berlin 12351, Germany.
Pathogens. 2020 Jan 31;9(2):91. doi: 10.3390/pathogens9020091.
The ideal laboratory test to detect () should be sensitive, specific, easy to use, rapid, and affordable and should provide information about susceptibility to antimicrobial drugs. Currently, such a test is not available and presumably will not be in the near future. Thus, diagnosis of gonococcal infections presently includes application of different techniques to address these requirements. Microscopy may produce rapid results but lacks sensitivity in many cases (except symptomatic urogenital infections in males). Highest sensitivity to detect was shown for nucleic acid amplification technologies (NAATs), which, however, are less specific than culture. In addition, comprehensive analysis of antibiotic resistance is accomplished only by in vitro antimicrobial susceptibility testing of cultured isolates. As a light at the end of the tunnel, new developments of molecular techniques and microfluidic systems represent promising opportunities to design point-of-care tests for rapid detection of with high sensitivity and specificity, and there is reason to hope that such tests may also provide antimicrobial resistance data in the future.
用于检测（）的理想实验室检测方法应具备灵敏、特异、易用、快速且经济实惠的特点，并应能提供对抗菌药物敏感性的信息。目前，尚无此类检测方法，而且在可预见的未来可能也不会有。因此，目前淋病感染的诊断包括应用不同技术来满足这些要求。显微镜检查可能会得出快速结果，但在许多情况下缺乏敏感性（男性有症状的泌尿生殖系统感染除外）。核酸扩增技术（NAATs）对检测（）的敏感性最高，然而，其特异性低于培养法。此外，只有通过对培养分离株进行体外抗菌药物敏感性测试才能完成对抗生素耐药性的全面分析。作为希望之光，分子技术和微流控系统的新发展为设计用于快速检测（）的即时检测方法提供了有前景的机会，这些方法具有高灵敏度和特异性，而且有理由希望此类检测方法未来也能提供抗菌药物耐药性数据。
