Kapasi K, Chui B, Inman R D
Department of Immunology, University of Toronto, Canada.
Immunology. 1992 Nov;77(3):456-61.
The association between three major spondyloarthritic diseases, ankylosing spondylitis, Reiter's syndrome, and reactive arthritis, and the major histocompatibility complex (MHC) class 1 antigen HLA-B27 is well documented. The hypothesis of cross-reactivity between HLA-B27 and the antecedent infection-causing Gram-negative pathogens such as Salmonella, Shigella and Yersinia has been suggested by in vitro studies employing monoclonal antibodies. We have examined the possibility of such cross-reactivity in vivo using various rabbit immune sera and patient sera as the source of cross-reacting antibody. Mouse L cells were transfected with HLA-A3 or HLA-B27 and used as a source of antigen. Western blot analysis employing denatured antigen, FACS analysis employing native antigen and immunoprecipitation studies were undertaken to detect cross-reacting antibodies generated in vivo to HLA-B27 antigen. Antibodies generated in vivo by infection in patients or immunization in animals against arthritogenic bacteria did not demonstrate any cross-reactivity with HLA-B27 by any of the methods used. As defined by the humoral immune response, molecular mimicry appears unlikely to explain the role of B27 in the pathogenesis of reactive arthritis.
三种主要的脊柱关节病——强直性脊柱炎、赖特综合征和反应性关节炎，与主要组织相容性复合体（MHC）I类抗原HLA - B27之间的关联已有充分文献记载。采用单克隆抗体的体外研究提出了HLA - B27与引发感染的革兰氏阴性病原体（如沙门氏菌、志贺氏菌和耶尔森氏菌）之间存在交叉反应性的假说。我们使用各种兔免疫血清和患者血清作为交叉反应抗体的来源，研究了体内这种交叉反应性的可能性。用HLA - A3或HLA - B27转染小鼠L细胞，并将其用作抗原来源。采用变性抗原进行蛋白质印迹分析、采用天然抗原进行荧光激活细胞分选分析以及进行免疫沉淀研究，以检测体内产生的针对HLA - B27抗原的交叉反应抗体。通过患者感染或动物针对致关节炎细菌免疫在体内产生的抗体，采用所使用的任何方法均未显示与HLA - B27有任何交叉反应性。就体液免疫反应而言，分子模拟似乎不太可能解释B27在反应性关节炎发病机制中的作用。
