KAPLAN M H, DALLENBACH F D
J Exp Med. 1961 Jan 1;113(1):1-16. doi: 10.1084/jem.113.1.1.
Using fluorescent antibody methods, deposits of bound gamma globulin, as determined in unfixed washed sections of auricular appendages from rheumatic hearts, were noted in a significant number (18 per cent) of 100 specimens studied. Such deposits were observed in myofibers, sarcolemma, interstitial connective tissue, and vessel walls. Albumin and fibrin were generally found absent from these sites. Control hearts from normal and pathologic material, including postmortem and biopsied specimens, in general, did not reveal such deposits. These various tissue sites which contained bound gamma globulin frequently exhibited evidence of alteration as indicated both by enhanced affinity for eosin and by strongly positive reaction with the periodic acid-Schiff reagent, and appeared comparable in some cases to "fibrinoid." Bound gamma globulin was not observed in cellular or stromal components of Aschoff lesions, nor was the occurrence of Aschoff lesions correlated with presence of bound gamma globulin. It is suggested that deposition of gamma globulin and the eosinophilic alteration associated with such deposition are related to certain of the pathologic changes of rheumatic heart disease. The nature of such deposits of gamma globulin was considered from immune and non-immune points of view.
运用荧光抗体法，在对100个风湿性心脏病心耳附器未固定冲洗切片的研究中，发现大量样本（18%）存在结合γ球蛋白沉积。在肌纤维、肌膜、间质结缔组织和血管壁中均观察到此类沉积。这些部位通常未发现白蛋白和纤维蛋白。取自正常及病理材料（包括尸检和活检标本）的对照心脏，一般未发现此类沉积。这些含有结合γ球蛋白的不同组织部位，常表现出改变的迹象，如对伊红亲和力增强以及与过碘酸 - 希夫试剂呈强阳性反应，在某些情况下与“纤维蛋白样”表现相似。在阿绍夫小体的细胞或基质成分中未观察到结合γ球蛋白，且阿绍夫小体的出现与结合γ球蛋白的存在无关。提示γ球蛋白沉积以及与此类沉积相关的嗜酸性改变与风湿性心脏病的某些病理变化有关。从免疫和非免疫角度对γ球蛋白此类沉积的性质进行了探讨。
