Stanley J, Burnens A P, Linton D, On S L, Costas M, Owen R J
National Collection of Type Cultures, Central Public Health Laboratory, London, UK.
J Gen Microbiol. 1992 Nov;138(11):2293-303. doi: 10.1099/00221287-138-11-2293.
An atypical group of thermophilic catalase-negative Campylobacter strains, the 'CH' (Swiss) group, can be recovered from faeces of domestic cats and dogs after selection by filtration, or with the antibiotic cefoperazone. This group of strains shows no relative DNA homology with any species in rRNA superfamily VI (Vandamme et al., 1991, International Journal of Systematic Bacteriology 41, 88-103) except with four thermophilic Campylobacter species, notably C. upsaliensis. The group is homogeneous and possesses a DNA base composition, cellular morphology at the electron microscope level and phenotypic properties characteristic of Campylobacter. Nonetheless it is distinct from known species of Campylobacter in terms of conventional bacteriological tests, total cellular protein profile, rRNA gene profile, and genomic DNA homology. On the basis of an integrated study of phenotype and genotype, we conclude that these bacteria constitute a previously undescribed species for which we propose the name Campylobacter helveticus sp. nov. A species-specific recombinant DNA probe was cloned from the designated type strain (NCTC 12470) for use in identification and further analysis of the epidemiology, pathogenicity and transmission of C. helveticus.
一类非典型的嗜热过氧化氢酶阴性弯曲杆菌菌株，即“CH”（瑞士）菌群，可通过过滤或使用抗生素头孢哌酮筛选后，从家猫和家犬的粪便中分离得到。该菌群的菌株与rRNA超家族VI中的任何物种（Vandamme等人，1991年，《国际系统细菌学杂志》41卷，88 - 103页）均无相对DNA同源性，但与四种嗜热弯曲杆菌物种，特别是犬弯曲杆菌有同源性。该菌群具有同质性，拥有弯曲杆菌特有的DNA碱基组成、电子显微镜水平下的细胞形态和表型特性。然而，就传统细菌学检测、全细胞蛋白图谱、rRNA基因图谱和基因组DNA同源性而言，它与已知的弯曲杆菌物种不同。基于对表型和基因型的综合研究，我们得出结论，这些细菌构成了一个以前未描述的物种，我们提议将其命名为瑞士弯曲杆菌（Campylobacter helveticus sp. nov.）。从指定的模式菌株（NCTC 12470）中克隆了一种物种特异性重组DNA探针，用于瑞士弯曲杆菌的鉴定以及进一步分析其流行病学、致病性和传播情况。
