Rossi Mirko, Debruyne Lies, Zanoni Renato Giulio, Manfreda Gerardo, Revez Joana, Vandamme Peter
Department of Veterinary Public Health and Animal Pathology, Alma Mater Studiorum, University of Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia, Bologna, Italy.
Int J Syst Evol Microbiol. 2009 Sep;59(Pt 9):2364-9. doi: 10.1099/ijs.0.007419-0. Epub 2009 Jul 20.
Three strains of an unusual hippurate-positive Campylobacter species were isolated at 37 degrees C from caecal contents of broiler chickens and a turkey. All strains were initially identified as Campylobacter by means of genus-specific PCR, but none was further identified using specific PCRs for known thermophilic species. Phylogenetic analyses based on 16S rRNA, rpoB and groEL gene sequences revealed that these strains formed a robust clade distinct from other Campylobacter species. Amplified fragment length polymorphism analysis and whole-cell protein electrophoresis were subsequently carried out and confirmed the divergence between the avian strains and other taxa. These data indicate that the unidentified Campylobacter strains belong to a novel taxon which could be distinguished from other campylobacters through its phenotypic and genotypic characteristics. The name Campylobacter avium sp. nov., is proposed for the novel species, with the type strain 86/06T (=LMG 24591T=CCUG 56292T).
从肉鸡和一只火鸡的盲肠内容物中，在37摄氏度下分离出三株不寻常的马尿酸盐阳性弯曲杆菌。所有菌株最初通过属特异性PCR鉴定为弯曲杆菌，但使用已知嗜热菌种的特异性PCR均未进一步鉴定。基于16S rRNA、rpoB和groEL基因序列的系统发育分析表明，这些菌株形成了一个与其他弯曲杆菌物种不同的稳健分支。随后进行了扩增片段长度多态性分析和全细胞蛋白质电泳，证实了禽类菌株与其他分类群之间的差异。这些数据表明，未鉴定的弯曲杆菌菌株属于一个新的分类单元，可通过其表型和基因型特征与其他弯曲杆菌区分开来。建议将该新物种命名为鸟弯曲杆菌（Campylobacter avium sp. nov.），模式菌株为86/06T（=LMG 24591T=CCUG 56292T）。
