Shetty V P, Suchitra K, Uplekar M W, Antia N H
Foundation for Medical Research, Worli, Bombay, India.
Lepr Rev. 1992 Dec;63(4):329-36. doi: 10.5935/0305-7518.19920039.
Skin and nerve biopsies obtained from 18 multibacillary (MB) and 16 paucibacillary (PB) cases of leprosy who had been fully treated by the WHO regimen were assessed for bacterial load using different staining techniques. In addition skin and nerve homogenates of 10 MB cases were tested for 'persistor' Mycobacterium leprae using immunosuppressed mice. While significant amounts of integral bacilli and BCG cross-reactive antigen of M. leprae were detected both in skin and nerve tissues of all the MB cases (100%), 56% of skin and 62% of nerve biopsies of PB cases also showed the presence of BCG cross-reactive antigen. Detection of 'persistor' M. leprae in 2/10 skin biopsies (20%) and 3/10 nerve biopsies (30%) of MB cases was thought to be unexpectedly high after 2 years of MDT.
对18例多菌型（MB）和16例少菌型（PB）麻风病例的皮肤和神经活检样本进行评估，这些病例均已按照世界卫生组织方案完成治疗，采用不同染色技术检测细菌载量。此外，利用免疫抑制小鼠对10例MB病例的皮肤和神经匀浆进行检测，以寻找“持留菌”麻风分枝杆菌。虽然在所有MB病例（100%）的皮肤和神经组织中均检测到大量完整杆菌和麻风分枝杆菌的卡介苗交叉反应抗原，但PB病例的皮肤活检样本中有56%、神经活检样本中有62%也显示存在卡介苗交叉反应抗原。在接受多药联合化疗2年后，MB病例的10份皮肤活检样本中有2份（20%）、10份神经活检样本中有3份（3%）检测到“持留菌”麻风分枝杆菌，这一比例被认为出乎意料地高。
