Hsu E M, McNicol P J
Department of Medical Microbiology, University of Manitoba, Canada.
Mol Cell Probes. 1992 Dec;6(6):459-66. doi: 10.1016/0890-8508(92)90042-v.
Human papillomavirus (HPV) is associated with specific benign and malignant lesions of the epithelial and mucosal surfaces. Of the sexually transmitted types, HPV type 16 (HPV-16) is the most commonly associated with carcinoma of the uterine cervix. Expression of the E6/E7 open reading frame of the viral genome is considered critical in the development of neoplasia. Using the CaSki cervical carcinoma cell line as a model system, we have adapted the polymerase chain reaction to quantify the transcripts expressed from this region. It was found that 97.1% of the total spliced transcript is E6I, which putatively encodes the E7 oncoprotein, while E6II comprises 2.9% of spliced product. The ratio of E6I to E6II expression may be an important parameter in evaluating the disease risk associated with HPV-16 infection.
人乳头瘤病毒（HPV）与上皮和粘膜表面的特定良性及恶性病变相关。在性传播类型中，HPV 16型（HPV - 16）与子宫颈癌最为相关。病毒基因组E6/E7开放阅读框的表达被认为在肿瘤形成过程中至关重要。以CaSki宫颈癌细胞系作为模型系统，我们采用聚合酶链反应来定量该区域表达的转录本。结果发现，总剪接转录本的97.1%是E6I，其可能编码E7癌蛋白，而E6II占剪接产物的2.9%。E6I与E6II的表达比例可能是评估与HPV - 16感染相关疾病风险的一个重要参数。
