Winkler B S, Trese M T
Eye Research Institute, Oakland University, Rochester, MI 48309.
Ophthalmic Surg. 1992 Sep;23(9):622-4.
Experiments were undertaken to measure the pH of solutions of commonly used intravitreal antibiotics in various irrigating solutions to determine whether a change in pH might be a factor in antibiotic-induced retinal toxicity. Such retinal toxicity has been particularly damaging when solutions of gentamicin have been used. The pHs of the following solutions were measured: gentamicin, amikacin, methicillin, tobramycin, and vancomycin, combined with balanced salt solution (BSS) PLUS (bicarbonate buffer), BSS (citrate/acetate buffer), and lactated Ringer's solution (lactate buffer). Each of these antibiotics induced a concentration-dependent decrease in pH of the solutions; gentamicin, amikacin and tobramycin produced the largest shifts. The results also demonstrated that BSS PLUS acts as the strongest buffer and lactated Ringer's as the weakest. We conclude that it is important to determine the pH of intraocular antibiotic irrigating solutions, not just the pH of the antibiotics.
开展了实验以测量常用玻璃体内抗生素在各种冲洗液中的溶液pH值，以确定pH值变化是否可能是抗生素诱导视网膜毒性的一个因素。当使用庆大霉素溶液时，这种视网膜毒性尤其具有破坏性。测量了以下溶液的pH值：庆大霉素、阿米卡星、甲氧西林、妥布霉素和万古霉素，分别与平衡盐溶液（BSS）PLUS（碳酸氢盐缓冲液）、BSS（柠檬酸盐/醋酸盐缓冲液）和乳酸林格氏液（乳酸盐缓冲液）混合。这些抗生素中的每一种都会导致溶液pH值呈浓度依赖性下降；庆大霉素、阿米卡星和妥布霉素引起的变化最大。结果还表明，BSS PLUS是最强的缓冲液，而乳酸林格氏液是最弱的。我们得出结论，确定眼内抗生素冲洗液的pH值很重要，而不仅仅是抗生素的pH值。
