TATA 结合蛋白参与 tRNA 基因上 TFIIIB 的组装。

DBCM--Service de Biochimie et Génétique Moléculaire, Centre d'Etudes de Saclay, Gif-sur-Yvette, France.

Nucleic Acids Res. 1992 Dec 25;20(24):6451-4. doi: 10.1093/nar/20.24.6451.

The TATA-binding protein TBP has been recently recognized as a general class III transcription factor. Using the gel shift assay to monitor initiation complex assembly on a yeast tRNA gene, we show that TBP is required for the TFIIIC-dependent assembly of TFIIIB. TFIIIB depleted of TBP by a simple chromatographic step does not bind stably to the TFIIIC-tDNA complex. Addition of yeast or human recombinant TBP allows the formation of a TFIIIB-TBP-TFIIIC-tDNA complex. The presence of TBP in the complex was inferred from the effect of anti-TBP antibodies and from the different migration properties of TFIIIB-TBP-tDNA complexes formed with yeast or human TBP.

TATA 结合蛋白TBP最近被认为是一种通用的III类转录因子。通过凝胶迁移试验监测酵母tRNA基因上起始复合物的组装，我们发现TBP是TFIIIB依赖于TFIIIC组装所必需的。通过简单的色谱步骤去除TBP的TFIIIB不能稳定地结合到TFIIIC-tDNA复合物上。添加酵母或人重组TBP可形成TFIIIB-TBP-TFIIIC-tDNA复合物。根据抗TBP抗体的作用以及用酵母或人TBP形成的TFIIIB-TBP-tDNA复合物的不同迁移特性推断复合物中存在TBP。